Expression of a developmentally regulated gene, Mng10, in identified neurosecretory cells in the CNS of Manduca sexta

Melinda Mészáros, David Morton

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We are interested in the molecular events underlying the development of the nervous system of Manduca sexta during the final 24 h of the pupal molt. In this article we describe a gene, Mng10, that is expressed in the abdominal nervous system of M. sexta and is developmentally regulated over this 24-h period. In situ hybridization analysis shows that the transcript is localized predominantly to a single pair of uniquely identifiable neurosecretory neurons, the NS-L1 cells in the abdominal ganglia. Mng10 is a single copy gene encoding a 229 amino acid protein with a predicted molecular mass of 26 kDa. At the amino acid level the protein shows 34% identity to the yeast transcription unit, Yer082. Northern blot analysis shows that the transcript of Mng10 is very rare, comprising about 0.001% of the poly (A)+ RNA from the CNS and is detectable at 4 h but not 24 h prior to pupal ecdysis. One of the physiological events that develops over the final 24 h of the pupal molt is the ability of the nervous system to respond to the neuropeptide eclosion hormone. In this context, it is interesting to note that the NS-L1 cells are members of the group of 50 neurons that show increased cGMP immunoreactivity when the nervous system is exposed to the neuropeptide eclosion hormone.

Original languageEnglish (US)
Pages (from-to)349-358
Number of pages10
JournalJournal of Neurobiology
Volume30
Issue number3
DOIs
Publication statusPublished - Jul 1996
Externally publishedYes

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Keywords

  • ecdysis
  • eclosion hormone
  • neurosecretory cells
  • Yer082

ASJC Scopus subject areas

  • Neuroscience(all)

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