Expression and regulation of mPer1 in immortalized GnRH neurons

J. Olcese, R. Domagalski, A. Bednorz, D. R. Weaver, H. F. Urbanski, S. Reuss, R. Middendorff

    Research output: Contribution to journalArticle

    30 Scopus citations

    Abstract

    Hypothalamic GnRH (gonadotropin-releasing hormone) neurons play a critical role in the initiation and maintenance of reproduction competence. Using the mouse GnRH neuronal cell line, GT1-7, we have characterized the expression of the gene mPer1, a recognized key element of the mammalian circadian clockwork. Both mPer1 transcripts and the 136 kDa m PER1 gene product could be detected in these cells. Immunocytochemical analysis also confirmed expression of mPER1 both in vitro and in vivo in GnRH neurons. Activation of cyclic AMP signalling pathways in vitro elevated GnRH secretion as well as mPer1 expression and nuclear mPER1 immunoreactivity. As mPER1 is known to feedback on transcriptional activities in many cell models, the data presented here point to a role for mPER1 in the regulation of gene expression in GnRH neurons, and thus in the control of neuroendocrine activities.

    Original languageEnglish (US)
    Pages (from-to)613-618
    Number of pages6
    JournalNeuroReport
    Volume14
    Issue number4
    DOIs
    StatePublished - Mar 1 2003

    Keywords

    • Clock gene expression
    • GnRH neurons
    • cAMP
    • mPer1

    ASJC Scopus subject areas

    • Neuroscience(all)

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  • Cite this

    Olcese, J., Domagalski, R., Bednorz, A., Weaver, D. R., Urbanski, H. F., Reuss, S., & Middendorff, R. (2003). Expression and regulation of mPer1 in immortalized GnRH neurons. NeuroReport, 14(4), 613-618. https://doi.org/10.1097/00001756-200303240-00018