TY - JOUR
T1 - Expression and function of endothelial nitric oxide synthase messenger RNA and protein are higher in internal mammary than in radial arteries
AU - He, Guo Wei
AU - Fan, Li
AU - Grove, Kevin L.
AU - Furnary, Anthony
AU - Yang, Qin
N1 - Funding Information:
This study was supported by Providence St. Vincent Medical Foundation (Portland, OR), Tianjin Municipal Science and Technology Commission (09ZCZDSF04200 & 10JCYBJC26400), Ministry of Science and Technology, China (2009DFB30560 and 2010CB529502), Hong Kong RGC GRF grants ( CUHK4651/07M and CUHK4789/09M ), and CUHK direct grants 2041457 and 2041561.
PY - 2011/9
Y1 - 2011/9
N2 - Background: The internal mammary artery (IMA) has a better long-term patency rate than the radial artery (RA), but the underlying molecular mechanisms are unclear. We compared endothelial nitric oxide synthase (eNOS) and related NO release in these two arteries. Methods: Real-time polymerase chain reaction was used to quantify eNOS messenger RNA (mRNA) expression level in the endothelial cells of IMAs and RAs. eNOS protein localization was determined by immunohistochemistry. NO release from the endothelium of IMAs and RAs was directly measured by an electrochemical method using a membrane-type NO-sensitive electrode. Results: Endothelial nitric oxide synthase mRNA expression level was significantly higher in the endothelial cells of IMAs than in RAs (1.03 ± 0.19 vs 0.53 ± 0.09, n = 7, p < 0.05), but was similar in the whole vascular tissue. eNOS protein immunoreactivity was higher in the endothelial cells of IMAs than in RAs. NO release at both levels in IMAs was significantly greater than in RAs (basal: 17.5 ± 1.9 vs 10.2 ± 0.7 nM, n = 11 each, p = 0.003; stimulated with bradykinin -7 log M: 31.5 ± 3.6 vs 14.3 ± 5.3 nM, n = 6 each, p = 0.02). Conclusions: Endothelial cells in the IMA express higher levels of eNOS mRNA and protein than those in the RA, which is linked with higher release of NO. These findings may be related to the superior long-term patency rate of the IMA vs the RA. This study also provides some basic genetic information for grafting arteries.
AB - Background: The internal mammary artery (IMA) has a better long-term patency rate than the radial artery (RA), but the underlying molecular mechanisms are unclear. We compared endothelial nitric oxide synthase (eNOS) and related NO release in these two arteries. Methods: Real-time polymerase chain reaction was used to quantify eNOS messenger RNA (mRNA) expression level in the endothelial cells of IMAs and RAs. eNOS protein localization was determined by immunohistochemistry. NO release from the endothelium of IMAs and RAs was directly measured by an electrochemical method using a membrane-type NO-sensitive electrode. Results: Endothelial nitric oxide synthase mRNA expression level was significantly higher in the endothelial cells of IMAs than in RAs (1.03 ± 0.19 vs 0.53 ± 0.09, n = 7, p < 0.05), but was similar in the whole vascular tissue. eNOS protein immunoreactivity was higher in the endothelial cells of IMAs than in RAs. NO release at both levels in IMAs was significantly greater than in RAs (basal: 17.5 ± 1.9 vs 10.2 ± 0.7 nM, n = 11 each, p = 0.003; stimulated with bradykinin -7 log M: 31.5 ± 3.6 vs 14.3 ± 5.3 nM, n = 6 each, p = 0.02). Conclusions: Endothelial cells in the IMA express higher levels of eNOS mRNA and protein than those in the RA, which is linked with higher release of NO. These findings may be related to the superior long-term patency rate of the IMA vs the RA. This study also provides some basic genetic information for grafting arteries.
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U2 - 10.1016/j.athoracsur.2011.04.063
DO - 10.1016/j.athoracsur.2011.04.063
M3 - Article
C2 - 21871268
AN - SCOPUS:80052817863
SN - 0003-4975
VL - 92
SP - 845
EP - 850
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
IS - 3
ER -