Expression and function of endothelial nitric oxide synthase messenger RNA and protein are higher in internal mammary than in radial arteries

Guo Wei He, Li Fan, Kevin Grove, Anthony Furnary, Qin Yang

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    Background: The internal mammary artery (IMA) has a better long-term patency rate than the radial artery (RA), but the underlying molecular mechanisms are unclear. We compared endothelial nitric oxide synthase (eNOS) and related NO release in these two arteries. Methods: Real-time polymerase chain reaction was used to quantify eNOS messenger RNA (mRNA) expression level in the endothelial cells of IMAs and RAs. eNOS protein localization was determined by immunohistochemistry. NO release from the endothelium of IMAs and RAs was directly measured by an electrochemical method using a membrane-type NO-sensitive electrode. Results: Endothelial nitric oxide synthase mRNA expression level was significantly higher in the endothelial cells of IMAs than in RAs (1.03 ± 0.19 vs 0.53 ± 0.09, n = 7, p <0.05), but was similar in the whole vascular tissue. eNOS protein immunoreactivity was higher in the endothelial cells of IMAs than in RAs. NO release at both levels in IMAs was significantly greater than in RAs (basal: 17.5 ± 1.9 vs 10.2 ± 0.7 nM, n = 11 each, p = 0.003; stimulated with bradykinin -7 log M: 31.5 ± 3.6 vs 14.3 ± 5.3 nM, n = 6 each, p = 0.02). Conclusions: Endothelial cells in the IMA express higher levels of eNOS mRNA and protein than those in the RA, which is linked with higher release of NO. These findings may be related to the superior long-term patency rate of the IMA vs the RA. This study also provides some basic genetic information for grafting arteries.

    Original languageEnglish (US)
    Pages (from-to)845-850
    Number of pages6
    JournalAnnals of Thoracic Surgery
    Volume92
    Issue number3
    DOIs
    StatePublished - Sep 2011

    Fingerprint

    Radial Artery
    Nitric Oxide Synthase Type III
    Breast
    Messenger RNA
    Mammary Arteries
    Endothelial Cells
    Proteins
    Arteries
    Bradykinin
    Endothelium
    Blood Vessels
    Real-Time Polymerase Chain Reaction
    Electrodes
    Immunohistochemistry
    Membranes

    ASJC Scopus subject areas

    • Cardiology and Cardiovascular Medicine
    • Surgery
    • Pulmonary and Respiratory Medicine

    Cite this

    Expression and function of endothelial nitric oxide synthase messenger RNA and protein are higher in internal mammary than in radial arteries. / He, Guo Wei; Fan, Li; Grove, Kevin; Furnary, Anthony; Yang, Qin.

    In: Annals of Thoracic Surgery, Vol. 92, No. 3, 09.2011, p. 845-850.

    Research output: Contribution to journalArticle

    He, Guo Wei ; Fan, Li ; Grove, Kevin ; Furnary, Anthony ; Yang, Qin. / Expression and function of endothelial nitric oxide synthase messenger RNA and protein are higher in internal mammary than in radial arteries. In: Annals of Thoracic Surgery. 2011 ; Vol. 92, No. 3. pp. 845-850.
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    abstract = "Background: The internal mammary artery (IMA) has a better long-term patency rate than the radial artery (RA), but the underlying molecular mechanisms are unclear. We compared endothelial nitric oxide synthase (eNOS) and related NO release in these two arteries. Methods: Real-time polymerase chain reaction was used to quantify eNOS messenger RNA (mRNA) expression level in the endothelial cells of IMAs and RAs. eNOS protein localization was determined by immunohistochemistry. NO release from the endothelium of IMAs and RAs was directly measured by an electrochemical method using a membrane-type NO-sensitive electrode. Results: Endothelial nitric oxide synthase mRNA expression level was significantly higher in the endothelial cells of IMAs than in RAs (1.03 ± 0.19 vs 0.53 ± 0.09, n = 7, p <0.05), but was similar in the whole vascular tissue. eNOS protein immunoreactivity was higher in the endothelial cells of IMAs than in RAs. NO release at both levels in IMAs was significantly greater than in RAs (basal: 17.5 ± 1.9 vs 10.2 ± 0.7 nM, n = 11 each, p = 0.003; stimulated with bradykinin -7 log M: 31.5 ± 3.6 vs 14.3 ± 5.3 nM, n = 6 each, p = 0.02). Conclusions: Endothelial cells in the IMA express higher levels of eNOS mRNA and protein than those in the RA, which is linked with higher release of NO. These findings may be related to the superior long-term patency rate of the IMA vs the RA. This study also provides some basic genetic information for grafting arteries.",
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    AU - Grove, Kevin

    AU - Furnary, Anthony

    AU - Yang, Qin

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    N2 - Background: The internal mammary artery (IMA) has a better long-term patency rate than the radial artery (RA), but the underlying molecular mechanisms are unclear. We compared endothelial nitric oxide synthase (eNOS) and related NO release in these two arteries. Methods: Real-time polymerase chain reaction was used to quantify eNOS messenger RNA (mRNA) expression level in the endothelial cells of IMAs and RAs. eNOS protein localization was determined by immunohistochemistry. NO release from the endothelium of IMAs and RAs was directly measured by an electrochemical method using a membrane-type NO-sensitive electrode. Results: Endothelial nitric oxide synthase mRNA expression level was significantly higher in the endothelial cells of IMAs than in RAs (1.03 ± 0.19 vs 0.53 ± 0.09, n = 7, p <0.05), but was similar in the whole vascular tissue. eNOS protein immunoreactivity was higher in the endothelial cells of IMAs than in RAs. NO release at both levels in IMAs was significantly greater than in RAs (basal: 17.5 ± 1.9 vs 10.2 ± 0.7 nM, n = 11 each, p = 0.003; stimulated with bradykinin -7 log M: 31.5 ± 3.6 vs 14.3 ± 5.3 nM, n = 6 each, p = 0.02). Conclusions: Endothelial cells in the IMA express higher levels of eNOS mRNA and protein than those in the RA, which is linked with higher release of NO. These findings may be related to the superior long-term patency rate of the IMA vs the RA. This study also provides some basic genetic information for grafting arteries.

    AB - Background: The internal mammary artery (IMA) has a better long-term patency rate than the radial artery (RA), but the underlying molecular mechanisms are unclear. We compared endothelial nitric oxide synthase (eNOS) and related NO release in these two arteries. Methods: Real-time polymerase chain reaction was used to quantify eNOS messenger RNA (mRNA) expression level in the endothelial cells of IMAs and RAs. eNOS protein localization was determined by immunohistochemistry. NO release from the endothelium of IMAs and RAs was directly measured by an electrochemical method using a membrane-type NO-sensitive electrode. Results: Endothelial nitric oxide synthase mRNA expression level was significantly higher in the endothelial cells of IMAs than in RAs (1.03 ± 0.19 vs 0.53 ± 0.09, n = 7, p <0.05), but was similar in the whole vascular tissue. eNOS protein immunoreactivity was higher in the endothelial cells of IMAs than in RAs. NO release at both levels in IMAs was significantly greater than in RAs (basal: 17.5 ± 1.9 vs 10.2 ± 0.7 nM, n = 11 each, p = 0.003; stimulated with bradykinin -7 log M: 31.5 ± 3.6 vs 14.3 ± 5.3 nM, n = 6 each, p = 0.02). Conclusions: Endothelial cells in the IMA express higher levels of eNOS mRNA and protein than those in the RA, which is linked with higher release of NO. These findings may be related to the superior long-term patency rate of the IMA vs the RA. This study also provides some basic genetic information for grafting arteries.

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