Expressed sequence tags from the long arm of human chromosome 21

Pei Wen Chiang; Grzegorz Dzida; Jordan Grumet; Jan Fang Cheng; Woo Joo Song; Eric Crombez; Margaret L. Vankeuren; David M. Kurnit

doi:10.1006/geno.1995.9991

Expressed sequence tags from the long arm of human chromosome 21

Pei Wen Chiang, Grzegorz Dzida, Jordan Grumet, Jan Fang Cheng, Woo Joo Song, Eric Crombez, Margaret L. Vankeuren, David M. Kurnit

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We isolated expressed sequence tags (ESTs) on the long arm of chromosome 21. The ESTs were mapped by PCR using a monochromosomal somatic-cell mapping panel. Of a total of 55 cDNAs, 30 mapped back uniquely to chromosome 21, 7 mapped back to other chromosomes including chromosome 21, 8 mapped back to chromosomes other than 21, and 10 could not be assigned using this methodology. The 30 chromosome 21-specific markers so isolated represent useful EST markers. A rapid PCR-based method was used to delineate the expression pattern of these 30 pairs in different tissues.

Original language	English (US)
Pages (from-to)	383-389
Number of pages	7
Journal	Genomics
Volume	29
Issue number	2
DOIs	https://doi.org/10.1006/geno.1995.9991
State	Published - Sep 1995
Externally published	Yes

ASJC Scopus subject areas

Genetics

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10.1006/geno.1995.9991

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title = "Expressed sequence tags from the long arm of human chromosome 21",

abstract = "We isolated expressed sequence tags (ESTs) on the long arm of chromosome 21. The ESTs were mapped by PCR using a monochromosomal somatic-cell mapping panel. Of a total of 55 cDNAs, 30 mapped back uniquely to chromosome 21, 7 mapped back to other chromosomes including chromosome 21, 8 mapped back to chromosomes other than 21, and 10 could not be assigned using this methodology. The 30 chromosome 21-specific markers so isolated represent useful EST markers. A rapid PCR-based method was used to delineate the expression pattern of these 30 pairs in different tissues.",

author = "Chiang, {Pei Wen} and Grzegorz Dzida and Jordan Grumet and Cheng, {Jan Fang} and Song, {Woo Joo} and Eric Crombez and Vankeuren, {Margaret L.} and Kurnit, {David M.}",

note = "Funding Information: This work was supported by NIH Grants 1 R01 HL50025 and 1 R01 HG00923, by the American Heart Association, and by the Howard Hughes Medical Institute. D.M.K. is an Investigator and P.-W.C. is an Associate of the Howard Hughes Medical Institute.",

year = "1995",

month = sep,

doi = "10.1006/geno.1995.9991",

language = "English (US)",

volume = "29",

pages = "383--389",

journal = "Genomics",

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publisher = "Academic Press Inc.",

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T1 - Expressed sequence tags from the long arm of human chromosome 21

AU - Chiang, Pei Wen

AU - Dzida, Grzegorz

AU - Grumet, Jordan

AU - Cheng, Jan Fang

AU - Song, Woo Joo

AU - Crombez, Eric

AU - Vankeuren, Margaret L.

AU - Kurnit, David M.

N1 - Funding Information: This work was supported by NIH Grants 1 R01 HL50025 and 1 R01 HG00923, by the American Heart Association, and by the Howard Hughes Medical Institute. D.M.K. is an Investigator and P.-W.C. is an Associate of the Howard Hughes Medical Institute.

PY - 1995/9

Y1 - 1995/9

N2 - We isolated expressed sequence tags (ESTs) on the long arm of chromosome 21. The ESTs were mapped by PCR using a monochromosomal somatic-cell mapping panel. Of a total of 55 cDNAs, 30 mapped back uniquely to chromosome 21, 7 mapped back to other chromosomes including chromosome 21, 8 mapped back to chromosomes other than 21, and 10 could not be assigned using this methodology. The 30 chromosome 21-specific markers so isolated represent useful EST markers. A rapid PCR-based method was used to delineate the expression pattern of these 30 pairs in different tissues.

AB - We isolated expressed sequence tags (ESTs) on the long arm of chromosome 21. The ESTs were mapped by PCR using a monochromosomal somatic-cell mapping panel. Of a total of 55 cDNAs, 30 mapped back uniquely to chromosome 21, 7 mapped back to other chromosomes including chromosome 21, 8 mapped back to chromosomes other than 21, and 10 could not be assigned using this methodology. The 30 chromosome 21-specific markers so isolated represent useful EST markers. A rapid PCR-based method was used to delineate the expression pattern of these 30 pairs in different tissues.

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Expressed sequence tags from the long arm of human chromosome 21

Abstract

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