Experimenter effects on behavioral test scores of eight inbred mouse strains under the influence of ethanol

Martin Bohlen, Erika R. Hayes, Benjamin Bohlen, Jeremy D. Bailoo, John C. Crabbe, Douglas Wahlsten

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Eight standard inbred mouse strains were evaluated for ethanol effects on a refined battery of behavioral tests in a study that was originally designed to assess the influence of rat odors in the colony on mouse behaviors. As part of the design of the study, two experimenters conducted the tests, and the study was carefully balanced so that equal numbers of mice in all groups and times of day were tested by each experimenter. A defect in airflow in the facility compromised the odor manipulation, and in fact the different odor exposure groups did not differ in their behaviors. The two experimenters, however, obtained markedly different results for three of the tests. Certain of the experimenter effects arose from the way they judged behaviors that were not automated and had to be rated by the experimenter, such as slips on the balance beam. Others were not evident prior to ethanol injection but had a major influence after the injection. For several measures, the experimenter effects were notably different for different inbred strains. Methods to evaluate and reduce the impact of experimenter effects in future research are discussed.

Original languageEnglish (US)
Pages (from-to)46-54
Number of pages9
JournalBehavioural Brain Research
Volume272
DOIs
StatePublished - Oct 1 2014

Keywords

  • Accelerating rotarod
  • Balance beam
  • Grip strength
  • Laboratory environment
  • Motor coordination
  • Open field activity

ASJC Scopus subject areas

  • Behavioral Neuroscience

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