Experimental stroke induces massive, rapid activation of the peripheral immune system

Halina Offner, Sandhya Subramanian, Susan M. Parker, Michael E. Afentoulis, Arthur Vandenbark, Patricia D. Hurn

Research output: Contribution to journalArticle

351 Citations (Scopus)

Abstract

Clinical experimental stroke induces injurious local brain inflammation. However, effects on the peripheral immune system have not been well characterized. We quantified mRNA and protein levels for cytokines, chemokines, and chemokine receptors (CCR) in brain, spinal cord, peripheral lymphoid organs (spleen, lymph node, blood, and cultured mononuclear cells from these sources), and blood plasma after reversible middle cerebral artery occlusion (MCAO) or sham treatment in male C57BL/6 mice. Middle cerebral artery occlusion induced a complex, but organ specific, pattern of inflammatory factors in the periphery. At both 6 and 22 h after MCAO, activated spleen cells from stroke-injured mice secreted significantly enhanced levels of TNF-α, IFN-γ, IL-6, MCP-1, and IL-2. Unstimulated splenocytes expressed increased chemokines and CCR, including MIP-2 and CCR2, CCR7 & CCR8 at 6 h; and MIP-2, IP-10, and CCR1 & CCR2 at 22 h. Also at 22 h, T cells from blood and lymph nodes secreted increased levels of inflammatory cytokines after activation. As expected, there were striking proinflammatory changes in postischemic brain. In contrast, spinal cord displayed suppression of all mediators, suggesting a compensatory response to intracranial events. These data show for the first time that focal cerebral ischemia results in dynamic and widespread activation of inflammatory cytokines, chemokines, and CCR in the peripheral immune system.

Original languageEnglish (US)
Pages (from-to)654-665
Number of pages12
JournalJournal of Cerebral Blood Flow and Metabolism
Volume26
Issue number5
DOIs
StatePublished - May 2006

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Chemokines
Immune System
Stroke
Chemokine Receptors
Middle Cerebral Artery Infarction
Cytokines
Spinal Cord
Spleen
Lymph Nodes
Brain
Encephalitis
Brain Ischemia
Inbred C57BL Mouse
Interleukin-2
Cultured Cells
Interleukin-6
Placebos
T-Lymphocytes
Messenger RNA
Proteins

Keywords

  • Chemokines
  • Cytokines
  • Peripheral immunity
  • Receptors
  • Stroke

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Experimental stroke induces massive, rapid activation of the peripheral immune system. / Offner, Halina; Subramanian, Sandhya; Parker, Susan M.; Afentoulis, Michael E.; Vandenbark, Arthur; Hurn, Patricia D.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 26, No. 5, 05.2006, p. 654-665.

Research output: Contribution to journalArticle

Offner, Halina ; Subramanian, Sandhya ; Parker, Susan M. ; Afentoulis, Michael E. ; Vandenbark, Arthur ; Hurn, Patricia D. / Experimental stroke induces massive, rapid activation of the peripheral immune system. In: Journal of Cerebral Blood Flow and Metabolism. 2006 ; Vol. 26, No. 5. pp. 654-665.
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