Experimental stroke in the female diabetic, db/db, mouse

Susan J. Vannucci, Lisa B. Willing, Shozo Goto, Nabil Alkayed, Robert M. Brucklacher, Teresa L. Wood, Javad Towfighi, Patricia D. Hurn, Ian A. Simpson

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Diabetic hyperglycemia increases brain damage after cerebral ischemia in animals and humans, although the underlying mechanisms remain unclear. Gender-linked differences in ischemic tolerance have been described but have not been studied in the context of diabetes. In the current study, we used a model of unilateral common carotid artery ligation, combined with systemic hypoxia, to study the effects of diabetes and gender on hypoxic-ischemic (HI) brain damage in the genetic model of Type II diabetes, the db/db, mouse. Male and female, control and db/db, mice were subjected to right common carotid artery ligation followed by varying periods of hypoxia (8% oxygen/92% nitrogen) to assess mortality, infarct volume, and tissue damage by light microscopic techniques, Endischemic regional cerebral blood flow (CBF) was determined using [14C] iodoantipyrine autoradiography. Glycolytic and high energy phosphate compounds were measured in blood and brain by enzymatic and fluorometric techniques. Gender and diabetes had significant effects on mortality from HI and extent of brain damage in the survivors. Female mice were more resistant than their male counterparts, such that the severity (mortality and infarction size) in the male diabetics > female diabetics ∼ male controls > female controls. Endischemic CBF and depletion of cerebral high energy reserves were comparable among all groups. Surprisingly, female diabetic mice were more hyperglycemic and demonstrated a greater prolonged lactacidosis than the males; however, they were more resistant to damage. The results suggest a unique pathophysiology of hypoxia-ischemia in the female diabetic brain.

Original languageEnglish (US)
Pages (from-to)52-60
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume21
Issue number1
StatePublished - 2001
Externally publishedYes

Fingerprint

Stroke
Cerebrovascular Circulation
Common Carotid Artery
Brain
Ligation
Mortality
Brain Hypoxia
Genetic Models
Regional Blood Flow
Brain Ischemia
Autoradiography
Hyperglycemia
Type 2 Diabetes Mellitus
Infarction
Survivors
Nitrogen
Ischemia
Phosphates
Oxygen
Light

Keywords

  • Brain
  • Cerebral blood flow
  • Diabetes
  • Estrogen
  • Hyperglycemia
  • Stroke

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Vannucci, S. J., Willing, L. B., Goto, S., Alkayed, N., Brucklacher, R. M., Wood, T. L., ... Simpson, I. A. (2001). Experimental stroke in the female diabetic, db/db, mouse. Journal of Cerebral Blood Flow and Metabolism, 21(1), 52-60.

Experimental stroke in the female diabetic, db/db, mouse. / Vannucci, Susan J.; Willing, Lisa B.; Goto, Shozo; Alkayed, Nabil; Brucklacher, Robert M.; Wood, Teresa L.; Towfighi, Javad; Hurn, Patricia D.; Simpson, Ian A.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 21, No. 1, 2001, p. 52-60.

Research output: Contribution to journalArticle

Vannucci, SJ, Willing, LB, Goto, S, Alkayed, N, Brucklacher, RM, Wood, TL, Towfighi, J, Hurn, PD & Simpson, IA 2001, 'Experimental stroke in the female diabetic, db/db, mouse', Journal of Cerebral Blood Flow and Metabolism, vol. 21, no. 1, pp. 52-60.
Vannucci SJ, Willing LB, Goto S, Alkayed N, Brucklacher RM, Wood TL et al. Experimental stroke in the female diabetic, db/db, mouse. Journal of Cerebral Blood Flow and Metabolism. 2001;21(1):52-60.
Vannucci, Susan J. ; Willing, Lisa B. ; Goto, Shozo ; Alkayed, Nabil ; Brucklacher, Robert M. ; Wood, Teresa L. ; Towfighi, Javad ; Hurn, Patricia D. ; Simpson, Ian A. / Experimental stroke in the female diabetic, db/db, mouse. In: Journal of Cerebral Blood Flow and Metabolism. 2001 ; Vol. 21, No. 1. pp. 52-60.
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