Experimental otitis media with effusion induced by platelet activating factor

Chung Ku Rhee, Timothy T.K. Jung, Stanley Miller, Douglas Weeks

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

This study tested the hypothesis that platelet activating factor (PAF) in the middle ear can induce otitis media with effusion (OME) and that PAF antagonists can prevent PAF-induced OME. An initial trial of 16 μg of PAF was injected into chinchilla bullae, and all ears developed middle ear effusion (MEE) within 48 hours. Subsequent trials were performed to test dose dependency. Interestingly, 1 or 16 μg of PAF caused more MEE and inflammation than did 4 or 8 μg. A dose of 0.5 μg PAF did not cause MEE. Middle ear effusion from injected bullae contained the full spectrum of lipoxygenase and cyclooxygenase products; additionally, more PAF was detected than was injected. Finally, a PAF antagonist (WEB 2170) injected intraperitoneally prevented PAF-induced OME. This study demonstrates that PAF injected into the middle ear can induce OME and that PAF antagonists effectively prevent PAF-induced OME. These findings suggest that PAF plays an important role in the pathogenesis of OME.

Original languageEnglish (US)
Pages (from-to)600-605
Number of pages6
JournalAnnals of Otology, Rhinology & Laryngology
Volume102
Issue number8
DOIs
StatePublished - Aug 1993

Keywords

  • PAF antagonist
  • otitis media with effusion
  • platelet activating factor

ASJC Scopus subject areas

  • Otorhinolaryngology

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