Experimental otitis media with effusion induced by platelet activating factor

C. K. Rhee, T. T K Jung, S. Miller, Douglas Weeks

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

This study tested the hypothesis that platelet activating factor (PAF) in the middle ear can induce otitis media with effusion (OME) and that PAF antagonists can prevent PAF-induced OME. An initial trial of 16 μg of PAF was injected into chinchilla bullae, and all ears developed middle ear effusion (MEE) within 48 hours. Subsequent trials were performed to test dose dependency. Interestingly, 1 or 16 μg of PAF caused more MEE and inflammation than did 4 or 8 μg. A dose of 0.5 μg PAF did not cause MEE. Middle ear effusion from injected bullae contained the full spectrum of lipoxygenase and cyclooxygenase products; additionally, more PAF was detected than was injected. Finally, a PAF antagonist (WEB 2170) injected intraperitoneally prevented PAF-induced OME. This study demonstrates that PAF injected into the middle ear can induce OME and that PAF antagonists effectively prevent PAF-induced OME. These findings suggest that PAF plays an important role in the pathogenesis of OME.

Original languageEnglish (US)
Pages (from-to)600-605
Number of pages6
JournalAnnals of Otology, Rhinology and Laryngology
Volume102
Issue number8 I
StatePublished - 1993
Externally publishedYes

Fingerprint

Otitis Media with Effusion
Platelet Activating Factor
Middle Ear
Blister
Chinchilla
Lipoxygenase
Otitis Media
Prostaglandin-Endoperoxide Synthases
Ear

Keywords

  • otitis media with effusion
  • PAF antagonist
  • platelet activating factor

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Rhee, C. K., Jung, T. T. K., Miller, S., & Weeks, D. (1993). Experimental otitis media with effusion induced by platelet activating factor. Annals of Otology, Rhinology and Laryngology, 102(8 I), 600-605.

Experimental otitis media with effusion induced by platelet activating factor. / Rhee, C. K.; Jung, T. T K; Miller, S.; Weeks, Douglas.

In: Annals of Otology, Rhinology and Laryngology, Vol. 102, No. 8 I, 1993, p. 600-605.

Research output: Contribution to journalArticle

Rhee, CK, Jung, TTK, Miller, S & Weeks, D 1993, 'Experimental otitis media with effusion induced by platelet activating factor', Annals of Otology, Rhinology and Laryngology, vol. 102, no. 8 I, pp. 600-605.
Rhee, C. K. ; Jung, T. T K ; Miller, S. ; Weeks, Douglas. / Experimental otitis media with effusion induced by platelet activating factor. In: Annals of Otology, Rhinology and Laryngology. 1993 ; Vol. 102, No. 8 I. pp. 600-605.
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