Experimental exposure of zebrafish, Danio rerio (Hamilton), to Mycobacterium marinum and Mycobacterium peregrinum reveals the gastrointestinal tract as the primary route of infection: A potential model for environmental mycobacterial infection

Melanie Harriff, L. E. Bermudez, M. L. Kent

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The natural route by which fish become infected with mycobacteria is unknown. Danio rerio (Hamilton) were exposed by bath immersion and intubation to Mycobacterium marinum and Mycobacterium peregrinum isolates obtained from diseased zebrafish. Exposed fish were collected over the course of 8 weeks and examined for the presence of mycobacteriosis. Mycobacteria were consistently cultured from the intestines, and often from the livers and spleens of fish exposed by both methods. Mycobacteria were not observed in the gills. Histological analysis revealed that fish infected with M. marinum often developed granulomas accompanied by clinical signs of mycobacteriosis, while infection with M. peregrinum infrequently led to clinical signs of disease. Passage of the bacteria through environmental amoebae (Acanthamoeba castellani) was associated with increased growth of M. peregrinum over the course of 8 weeks, when compared to infection with the bacteria not passed through amoebae. The results provide evidence that zebrafish acquire mycobacteria primarily through the intestinal tract, resulting in mycobacterial dissemination.

Original languageEnglish (US)
Pages (from-to)587-600
Number of pages14
JournalJournal of Fish Diseases
Volume30
Issue number10
DOIs
StatePublished - Oct 2007
Externally publishedYes

Fingerprint

Mycobacterium peregrinum
Mycobacterium marinum
environmental models
mycobacterial diseases
Mycobacterium
Zebrafish
Danio rerio
gastrointestinal system
Gastrointestinal Tract
Acanthamoeba
Fishes
fish
Infection
infection
intestines
bacterium
bacteria
granuloma
Bacteria
Immersion

Keywords

  • Model
  • Mycobacterium marinum
  • Mycobacterium peregrinum
  • Route of infection
  • Transmission
  • Zebrafish

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary(all)

Cite this

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abstract = "The natural route by which fish become infected with mycobacteria is unknown. Danio rerio (Hamilton) were exposed by bath immersion and intubation to Mycobacterium marinum and Mycobacterium peregrinum isolates obtained from diseased zebrafish. Exposed fish were collected over the course of 8 weeks and examined for the presence of mycobacteriosis. Mycobacteria were consistently cultured from the intestines, and often from the livers and spleens of fish exposed by both methods. Mycobacteria were not observed in the gills. Histological analysis revealed that fish infected with M. marinum often developed granulomas accompanied by clinical signs of mycobacteriosis, while infection with M. peregrinum infrequently led to clinical signs of disease. Passage of the bacteria through environmental amoebae (Acanthamoeba castellani) was associated with increased growth of M. peregrinum over the course of 8 weeks, when compared to infection with the bacteria not passed through amoebae. The results provide evidence that zebrafish acquire mycobacteria primarily through the intestinal tract, resulting in mycobacterial dissemination.",
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