Exercise protects against diet-induced insulin resistance through downregulation of protein kinase Cβ in mice

Xiaoquan Rao, Jixin Zhong, Xiaohua Xu, Brianna Jordan, Santosh Maurya, Zachary Braunstein, Tse Yao Wang, Wei Huang, Sudha Aggarwal, Muthu Periasamy, Sanjay Rajagopalan, Kamal Mehta, Qinghua Sun

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Physical exercise is an important and effective therapy for diabetes. However, its underlying mechanism is not fully understood. Protein kinase Cb (PKCβ) has been suggested to be involved in the pathogenesis of obesity and insulin resistance, but the role of PKCβ in exercise-induced improvements in insulin resistance is completely unknown. In this study, we evaluated the involvement of PKCβ in exercise-attenuated insulin resistance in high-fat diet (HFD)-fed mice. PKCβ-/- and wild-type mice were fed a HFD with or without exercise training. PKC protein expression, body and tissue weight change, glucose and insulin tolerance, metabolic rate, mitochondria size and number, adipose inflammation, and AKT activation were determined to evaluate insulin sensitivity and metabolic changes after intervention. PKCβ expression decreased in both skeletal muscle and liver tissue after exercise. Exercise and PKCβ deficiency can alleviate HFD-induced insulin resistance, as evidenced by improved insulin tolerance. In addition, fat accumulation and mitochondrial dysfunction induced by HFD were also ameliorated by both exercise and PKCβ deficiency. On the other hand, exercise had little effect on PKCβ-/- mice. Further, our data indicated improved activation of AKT, the downstream signal molecule of insulin, in skeletal muscle and liver of exercised mice, whereas PKCβ deficiency blunted the difference between sedentary and exercised mice. These results suggest that downregulation of PKCβ contributes to exercise-induced improvement of insulin resistance in HFD-fed mice.

Original languageEnglish (US)
Article numbere81364
JournalPloS one
Volume8
Issue number12
DOIs
StatePublished - Dec 9 2013
Externally publishedYes

Fingerprint

protein kinase C
Nutrition
insulin resistance
protein kinases
Protein Kinases
Protein Kinase C
Insulin Resistance
exercise
Down-Regulation
Insulin
Diet
mice
High Fat Diet
diet
high fat diet
Fats
Liver
Muscle
skeletal muscle
Skeletal Muscle

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Exercise protects against diet-induced insulin resistance through downregulation of protein kinase Cβ in mice. / Rao, Xiaoquan; Zhong, Jixin; Xu, Xiaohua; Jordan, Brianna; Maurya, Santosh; Braunstein, Zachary; Wang, Tse Yao; Huang, Wei; Aggarwal, Sudha; Periasamy, Muthu; Rajagopalan, Sanjay; Mehta, Kamal; Sun, Qinghua.

In: PloS one, Vol. 8, No. 12, e81364, 09.12.2013.

Research output: Contribution to journalArticle

Rao, X, Zhong, J, Xu, X, Jordan, B, Maurya, S, Braunstein, Z, Wang, TY, Huang, W, Aggarwal, S, Periasamy, M, Rajagopalan, S, Mehta, K & Sun, Q 2013, 'Exercise protects against diet-induced insulin resistance through downregulation of protein kinase Cβ in mice', PloS one, vol. 8, no. 12, e81364. https://doi.org/10.1371/journal.pone.0081364
Rao, Xiaoquan ; Zhong, Jixin ; Xu, Xiaohua ; Jordan, Brianna ; Maurya, Santosh ; Braunstein, Zachary ; Wang, Tse Yao ; Huang, Wei ; Aggarwal, Sudha ; Periasamy, Muthu ; Rajagopalan, Sanjay ; Mehta, Kamal ; Sun, Qinghua. / Exercise protects against diet-induced insulin resistance through downregulation of protein kinase Cβ in mice. In: PloS one. 2013 ; Vol. 8, No. 12.
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