Exclusive photorelease of signalling lipids at the plasma membrane

André Nadler, Dmytro A. Yushchenko, Rainer Müller, Frank Stein, Suihan Feng, Christophe Mulle, Mario Carta, Carsten Schultz

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Photoactivation of caged biomolecules has become a powerful approach to study cellular signalling events. Here we report a method for anchoring and uncaging biomolecules exclusively at the outer leaflet of the plasma membrane by employing a photocleavable, sulfonated coumarin derivative. The novel caging group allows quantifying the reaction progress and efficiency of uncaging reactions in a live-cell microscopy setup, thereby greatly improving the control of uncaging experiments. We synthesized arachidonic acid derivatives bearing the new negatively charged or a neutral, membrane-permeant coumarin caging group to locally induce signalling either at the plasma membrane or on internal membranes in β-cells and brain slices derived from C57B1/6 mice. Uncaging at the plasma membrane triggers a strong enhancement of calcium oscillations in β-cells and a pronounced potentiation of synaptic transmission while uncaging inside cells blocks calcium oscillations in β-cells and causes a more transient effect on neuronal transmission, respectively. The precise subcellular site of arachidonic acid release is therefore crucial for signalling outcome in two independent systems.

Original languageEnglish (US)
Article number10056
JournalNature Communications
Volume6
DOIs
StatePublished - Dec 21 2015
Externally publishedYes

Fingerprint

Cell membranes
lipids
Cell Membrane
Biomolecules
membranes
Lipids
Arachidonic Acid
Calcium Signaling
Bearings (structural)
cells
Cell signaling
Calcium
Membranes
Coumarins
calcium
Brain
Microscopic examination
oscillations
acids
Synaptic Transmission

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Nadler, A., Yushchenko, D. A., Müller, R., Stein, F., Feng, S., Mulle, C., ... Schultz, C. (2015). Exclusive photorelease of signalling lipids at the plasma membrane. Nature Communications, 6, [10056]. https://doi.org/10.1038/ncomms10056

Exclusive photorelease of signalling lipids at the plasma membrane. / Nadler, André; Yushchenko, Dmytro A.; Müller, Rainer; Stein, Frank; Feng, Suihan; Mulle, Christophe; Carta, Mario; Schultz, Carsten.

In: Nature Communications, Vol. 6, 10056, 21.12.2015.

Research output: Contribution to journalArticle

Nadler, A, Yushchenko, DA, Müller, R, Stein, F, Feng, S, Mulle, C, Carta, M & Schultz, C 2015, 'Exclusive photorelease of signalling lipids at the plasma membrane', Nature Communications, vol. 6, 10056. https://doi.org/10.1038/ncomms10056
Nadler A, Yushchenko DA, Müller R, Stein F, Feng S, Mulle C et al. Exclusive photorelease of signalling lipids at the plasma membrane. Nature Communications. 2015 Dec 21;6. 10056. https://doi.org/10.1038/ncomms10056
Nadler, André ; Yushchenko, Dmytro A. ; Müller, Rainer ; Stein, Frank ; Feng, Suihan ; Mulle, Christophe ; Carta, Mario ; Schultz, Carsten. / Exclusive photorelease of signalling lipids at the plasma membrane. In: Nature Communications. 2015 ; Vol. 6.
@article{8f855675439e4fee96f98379a0b69c4d,
title = "Exclusive photorelease of signalling lipids at the plasma membrane",
abstract = "Photoactivation of caged biomolecules has become a powerful approach to study cellular signalling events. Here we report a method for anchoring and uncaging biomolecules exclusively at the outer leaflet of the plasma membrane by employing a photocleavable, sulfonated coumarin derivative. The novel caging group allows quantifying the reaction progress and efficiency of uncaging reactions in a live-cell microscopy setup, thereby greatly improving the control of uncaging experiments. We synthesized arachidonic acid derivatives bearing the new negatively charged or a neutral, membrane-permeant coumarin caging group to locally induce signalling either at the plasma membrane or on internal membranes in β-cells and brain slices derived from C57B1/6 mice. Uncaging at the plasma membrane triggers a strong enhancement of calcium oscillations in β-cells and a pronounced potentiation of synaptic transmission while uncaging inside cells blocks calcium oscillations in β-cells and causes a more transient effect on neuronal transmission, respectively. The precise subcellular site of arachidonic acid release is therefore crucial for signalling outcome in two independent systems.",
author = "Andr{\'e} Nadler and Yushchenko, {Dmytro A.} and Rainer M{\"u}ller and Frank Stein and Suihan Feng and Christophe Mulle and Mario Carta and Carsten Schultz",
year = "2015",
month = "12",
day = "21",
doi = "10.1038/ncomms10056",
language = "English (US)",
volume = "6",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Exclusive photorelease of signalling lipids at the plasma membrane

AU - Nadler, André

AU - Yushchenko, Dmytro A.

AU - Müller, Rainer

AU - Stein, Frank

AU - Feng, Suihan

AU - Mulle, Christophe

AU - Carta, Mario

AU - Schultz, Carsten

PY - 2015/12/21

Y1 - 2015/12/21

N2 - Photoactivation of caged biomolecules has become a powerful approach to study cellular signalling events. Here we report a method for anchoring and uncaging biomolecules exclusively at the outer leaflet of the plasma membrane by employing a photocleavable, sulfonated coumarin derivative. The novel caging group allows quantifying the reaction progress and efficiency of uncaging reactions in a live-cell microscopy setup, thereby greatly improving the control of uncaging experiments. We synthesized arachidonic acid derivatives bearing the new negatively charged or a neutral, membrane-permeant coumarin caging group to locally induce signalling either at the plasma membrane or on internal membranes in β-cells and brain slices derived from C57B1/6 mice. Uncaging at the plasma membrane triggers a strong enhancement of calcium oscillations in β-cells and a pronounced potentiation of synaptic transmission while uncaging inside cells blocks calcium oscillations in β-cells and causes a more transient effect on neuronal transmission, respectively. The precise subcellular site of arachidonic acid release is therefore crucial for signalling outcome in two independent systems.

AB - Photoactivation of caged biomolecules has become a powerful approach to study cellular signalling events. Here we report a method for anchoring and uncaging biomolecules exclusively at the outer leaflet of the plasma membrane by employing a photocleavable, sulfonated coumarin derivative. The novel caging group allows quantifying the reaction progress and efficiency of uncaging reactions in a live-cell microscopy setup, thereby greatly improving the control of uncaging experiments. We synthesized arachidonic acid derivatives bearing the new negatively charged or a neutral, membrane-permeant coumarin caging group to locally induce signalling either at the plasma membrane or on internal membranes in β-cells and brain slices derived from C57B1/6 mice. Uncaging at the plasma membrane triggers a strong enhancement of calcium oscillations in β-cells and a pronounced potentiation of synaptic transmission while uncaging inside cells blocks calcium oscillations in β-cells and causes a more transient effect on neuronal transmission, respectively. The precise subcellular site of arachidonic acid release is therefore crucial for signalling outcome in two independent systems.

UR - http://www.scopus.com/inward/record.url?scp=84952045887&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84952045887&partnerID=8YFLogxK

U2 - 10.1038/ncomms10056

DO - 10.1038/ncomms10056

M3 - Article

C2 - 26686736

AN - SCOPUS:84952045887

VL - 6

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 10056

ER -