Excitatory postsynaptic potentials evoked by ventral root stimulation in neonate rat motoneurons in vitro

Zhi-Gen Jiang, E. Shen, M. Y. Wang, N. J. Dun

Research output: Contribution to journalArticle

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Abstract

Intracellular recordings were made from antidromically identified motoneurons in transverse (500 μm) lumbar spinal cord slices of neonatal (12-20 day) rats. Electrical stimulation of ventral rootlets evoked, with or without an antidromic spike or initial segment potential, a depolarizing response (latency, 1-4.2 ms), a hyperpolarizing response (latency, 1.5-3.5 ms), or a combination of two preceding responses in 38, 6, and 8% of motoneurons investigated. The hyperpolarizing response was reversibly eliminated by low Ca2+ (0.25 mM), d-tubocurarine (d-Tc; 10 μM) or strychnine (1 μM), suggesting that this response represents an inhibitory postsynaptic potential (IPSP) mediated by glycine or a related substance released from inhibitory interneurons subsequent to their activation by axon collaterals in a manner analogous to the Renshaw cell circuitry described for the cat motoneurons. The depolarizing responses were excitatory postsynaptic potentials (EPSPs), because they could be graded by varying the stimulus intensity and were reversibly abolished in low Ca2+ solution. Membrane hyperpolarization increased the amplitude of EPSPs, and the mean extrapolated reversal potential was -4 mV. EPSPs were augmented, rather than diminished, by dihydro-β-erythroidine (1 μM) or d-Tc, arguing against a role of recurrent motor axon collaterals in initiating the responses. The conduction velocity of the fibers initiating the EPSPs ranged from 0.35 to 0.96 m/s, indicating that these fibers were unmyelinated. Furthermore, the EPSP exhibited a constant delay when the stimulus frequency was varied from 1 to 5 Hz, and the synaptic delay estimated by extrapolation was

Original languageEnglish (US)
Pages (from-to)57-66
Number of pages10
JournalJournal of Neurophysiology
Volume65
Issue number1
StatePublished - 1991
Externally publishedYes

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Spinal Nerve Roots
Excitatory Postsynaptic Potentials
Motor Neurons
Reaction Time
Axons
Spinal Cord
Tubocurarine
Strychnine
Inhibitory Postsynaptic Potentials
Interneurons
Glycine
Electric Stimulation
Cats
In Vitro Techniques
Membranes

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Excitatory postsynaptic potentials evoked by ventral root stimulation in neonate rat motoneurons in vitro. / Jiang, Zhi-Gen; Shen, E.; Wang, M. Y.; Dun, N. J.

In: Journal of Neurophysiology, Vol. 65, No. 1, 1991, p. 57-66.

Research output: Contribution to journalArticle

Jiang, Zhi-Gen ; Shen, E. ; Wang, M. Y. ; Dun, N. J. / Excitatory postsynaptic potentials evoked by ventral root stimulation in neonate rat motoneurons in vitro. In: Journal of Neurophysiology. 1991 ; Vol. 65, No. 1. pp. 57-66.
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