Excitatory amino acids in rostral ventrolateral medulla support blood pressure during water deprivation in rats

Virginia Brooks, Korrina L. Freeman, Kathy A. Clow

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Water deprivation is associated with regional increases in sympathetic tone, but whether this is mediated by changes in brain stem regulation of sympathetic activity is unknown. Therefore, this study tested the hypothesis that water deprivation increases excitatory amino acid (EAA) drive of the rostral ventrolateral medulla (RVLM), by determining whether bilateral microinjection of kynurenate (Kyn; 2.7 nmol) into the RVLM decreases arterial pressure more in water-deprived than water-replete rats. Plasma osmolality was increased in 48-h water-deprived rats (313 ± 1 mosmol/kgH2O; P <0.05) compared with 24-h water-deprived rats (306 ± 2 mosmol/kgH2O) and water-replete animals (300 ± 2 mosmol/kgH2O). Kyn decreased arterial pressure by 28.1 ± 5.2 mmHg (P <0.01) in 48-h water-deprived rats but had no effect in water-replete rats (-5.9 ± 1.3 mmHg). Variable depressor effects were observed in 24-h water-deprived animals (-12.5 ± 2.4 mmHg, not significant); however, in all rats the Kyn depressor response was strongly correlated to the osmolality level (P <0.01; r2 = 0.47). The pressor responses to unilateral microinjection of increasing doses (0.1, 0.5, 1.0, and 5.0 nmol) of glutamate were enhanced (P <0.05) during water deprivation, but the pressor responses to intravenous phenylephrine injection were smaller (P <0.05). These data suggest that water deprivation increases EAA drive to the RVLM, in part by increasing responsiveness of the RVLM to EAA such as glutamate.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume286
Issue number5 55-5
DOIs
StatePublished - May 2004

Fingerprint

Water Deprivation
Excitatory Amino Acids
Blood Pressure
Water
Microinjections
Osmolar Concentration
Glutamic Acid
Arterial Pressure
Kynurenic Acid
Phenylephrine
Intravenous Injections
Brain Stem

Keywords

  • Arterial blood pressure
  • Brain
  • Glutamate
  • Kynurenic acid
  • Osmolality

ASJC Scopus subject areas

  • Physiology

Cite this

Excitatory amino acids in rostral ventrolateral medulla support blood pressure during water deprivation in rats. / Brooks, Virginia; Freeman, Korrina L.; Clow, Kathy A.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 286, No. 5 55-5, 05.2004.

Research output: Contribution to journalArticle

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