Excitatory amino acid receptors in the ventral tegmental area regulate dopamine release in the ventral striatum

Mignon Karreman, Ben H C Westerink, Bita Moghaddam

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

The role of excitatory amino acid (EAA) receptors located in the ventral tegmental area (VTA) in tonic and phasic regulation of dopamine release in the ventral striatum was investigated. Microdialysis in conscious rats was used to assess dopamine release primarily from the nucleus accumbens shell region of the ventral striatum while applying EAA antagonists or agonists to the VTA. Infusion of the AMPA/kainate receptor antagonist 6-cyano-7- nitroquinoxaline-2,3-dione (25 and 100 μM) into the VTA did not affect dopamine release in the ventral striatum. In contrast, intra-VTA infusion of the NMDA receptor antagonist 2-amino-5-phosphopentanoic acid (100 and 500 μM) dose-dependently decreased the striatal release of dopamine. Intra-VTA application of the ionotropic EAA receptor agonists NMDA and AMPA dose- dependently (10 and 100 μM) increased dopamine efflux in the ventral striatum. However, infusion of 50 or 500 μM trans-(±)-1-amino-1,3- cyclopentanedicarboxylic acid (ACPD), a metabotropic EAA receptor agonist, did not significantly affect these levels. These data suggest that NMDA receptors in the VTA exert a tonic excitatory influence on dopamine release in the ventral striatum. Furthermore, dopamine neurotransmission in this region may be enhanced by activation of NMDA and AMPA receptors, but not ACPD-sensitive metabotropic receptors, located in the VTA. These data further suggest that EAA regulation of dopamine release primarily occurs in the VTA as opposed to presynaptically at the terminal level.

Original languageEnglish (US)
Pages (from-to)601-607
Number of pages7
JournalJournal of Neurochemistry
Volume67
Issue number2
StatePublished - Aug 1996
Externally publishedYes

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Ventral Tegmental Area
Glutamate Receptors
Dopamine
Excitatory Amino Acid Agonists
N-Methyl-D-Aspartate Receptors
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
AMPA Receptors
6-Cyano-7-nitroquinoxaline-2,3-dione
Kainic Acid Receptors
Corpus Striatum
Excitatory Amino Acid Antagonists
Excitatory Amino Acids
Acids
Ventral Striatum
Microdialysis
Nucleus Accumbens
N-Methylaspartate
Synaptic Transmission
Rats
Chemical activation

Keywords

  • Dopamine
  • Glutamate
  • Microdialysis
  • Nucleus accumbens
  • Prefrontal cortex
  • Schizophrenia

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Excitatory amino acid receptors in the ventral tegmental area regulate dopamine release in the ventral striatum. / Karreman, Mignon; Westerink, Ben H C; Moghaddam, Bita.

In: Journal of Neurochemistry, Vol. 67, No. 2, 08.1996, p. 601-607.

Research output: Contribution to journalArticle

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