Excitation of rat locus coeruleus neurons by adenosine 5′-triphosphate: Ionic mechanism and receptor characterization

Ke-Zhong Shen, R. A. North

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

ATP and several congeners were applied to locus coeruleus neurons in slices cut from rat pons. Whole-cell recording of membrane current showed that ATP caused an inward current at -60 mV. Effective concentrations (applied by superfusion) were 3-300 μM, and the peak current was about 150 pA at -60 mV. 2-Methylthioadenosine 5′-triphosphate was slightly more potent than ATP, adenosine 5′-diphosphate was about equipotent with ATP, α,β-methylene adenosine 5′-triphosphate was slightly less potent than ATP, and β,γ′-methylene adenosine 5′-triphosphate and adenosine 5′-monophosphate had little or no effect. Adenosine (100 μM) caused small outward currents (40 pA). By changing the ionic composition of the pipette and extracellular solution, it was shown that the inward current resulted from both an increase in conductance to sodium ions and a reduction in conductance to potassium ions. It is concluded that rat locus coeruleus neurons express P2 purinoceptors, activation of which depolarizes the cells predominately by increasing a conductance that allows sodium ions to enter the cell.

Original languageEnglish (US)
Pages (from-to)894-899
Number of pages6
JournalJournal of Neuroscience
Volume13
Issue number3
StatePublished - 1993

Fingerprint

Locus Coeruleus
Adenosine Triphosphate
Neurons
Ions
Sodium
Purinergic P2 Receptors
Pons
Patch-Clamp Techniques
Adenosine Monophosphate
Adenosine
Adenosine Diphosphate
Potassium
Cell Membrane

Keywords

  • ATP
  • Locus coeruleus
  • P receptor
  • Potassium conductance
  • Purinoceptor
  • Sodium conductance

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Excitation of rat locus coeruleus neurons by adenosine 5′-triphosphate : Ionic mechanism and receptor characterization. / Shen, Ke-Zhong; North, R. A.

In: Journal of Neuroscience, Vol. 13, No. 3, 1993, p. 894-899.

Research output: Contribution to journalArticle

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AB - ATP and several congeners were applied to locus coeruleus neurons in slices cut from rat pons. Whole-cell recording of membrane current showed that ATP caused an inward current at -60 mV. Effective concentrations (applied by superfusion) were 3-300 μM, and the peak current was about 150 pA at -60 mV. 2-Methylthioadenosine 5′-triphosphate was slightly more potent than ATP, adenosine 5′-diphosphate was about equipotent with ATP, α,β-methylene adenosine 5′-triphosphate was slightly less potent than ATP, and β,γ′-methylene adenosine 5′-triphosphate and adenosine 5′-monophosphate had little or no effect. Adenosine (100 μM) caused small outward currents (40 pA). By changing the ionic composition of the pipette and extracellular solution, it was shown that the inward current resulted from both an increase in conductance to sodium ions and a reduction in conductance to potassium ions. It is concluded that rat locus coeruleus neurons express P2 purinoceptors, activation of which depolarizes the cells predominately by increasing a conductance that allows sodium ions to enter the cell.

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