Toluene treated cells were used for examining excision of pyrimidine dimers in E. coli strains W3110, DM845 (uvrA-), P3478 (polA-), and KS5064 (polA(ex1)). Excision occurring in toluene treated cells is rapid, adenosine 5' triphosphate dependent, and requires the uvrA gene function. In strains lacking either the polymerizing or 5'→ 3' exonucleolytic activity of dioxyribonucleic acid polymerase I, excision does occur. However, both in vivo and in vitro, the excision in such strains is initially slower than wild type.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of bacteriology|
|State||Published - Dec 1 1976|
ASJC Scopus subject areas
- Molecular Biology