Exchange-transfusion with pyridoxalated polymerized hemoglobin: Effects on liver PO2

M. Wehry, J. Penner, F. Campbell, Richard Mullins, R. Rink

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To examine the efficacy of modified stroma-free hemoglobin in maintaining liver PO2, rats were exchange-transfused to hematocrit 10% using pyridoxalated polymerized hemoglobin (plp-polyHb, 10-12 g/dl) prepared from crystalline Hb. Following hemodilution, plasma Hb was 7.4 g/dl, and rats were normotensive. Mean liver PO2 was 3.4 vs 23.3 mm Hg in sham-exchanged controls. Other rats, hemodiluted similarly with 6% albumin or hydroxyethylstarch, were hypotensive and died. At 24 hours plasma Hb was 2.0 g/dl, indicating an intravascular half-life of approximately 16 hours. Hepatic PO2 was 12.4 vs 26.8 mm Hg in nonhemodiluted controls. Data provided by clearance of low-dose indocyanine green suggested reduced plasma volume and depressed liver blood flow. Scattered foci of midzonal hypoxic damage were observed in liver lobules. The basis for hypoxic injury is considered to be due in part to the acute restriction of oxygen supply induced by exchange-transfusion with plp-polyHb. The rate of loss of intravascular hemoglobin and diminished plasma volume could have contributed to oxygen insufficiency as well. Endotoxin present in the plp-polyHb was not a factor.

Original languageEnglish (US)
Pages (from-to)249-261
Number of pages13
JournalCirculatory Shock
Volume23
Issue number4
StatePublished - 1987
Externally publishedYes

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Liver
Plasma Volume
Oxygen
Hemodilution
Indocyanine Green
Hematocrit
Endotoxins
Half-Life
Albumins
Hemoglobins
polyhemoglobin-pyridoxal-5-phosphate
Wounds and Injuries
stroma free hemoglobin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Wehry, M., Penner, J., Campbell, F., Mullins, R., & Rink, R. (1987). Exchange-transfusion with pyridoxalated polymerized hemoglobin: Effects on liver PO2. Circulatory Shock, 23(4), 249-261.

Exchange-transfusion with pyridoxalated polymerized hemoglobin : Effects on liver PO2. / Wehry, M.; Penner, J.; Campbell, F.; Mullins, Richard; Rink, R.

In: Circulatory Shock, Vol. 23, No. 4, 1987, p. 249-261.

Research output: Contribution to journalArticle

Wehry, M, Penner, J, Campbell, F, Mullins, R & Rink, R 1987, 'Exchange-transfusion with pyridoxalated polymerized hemoglobin: Effects on liver PO2', Circulatory Shock, vol. 23, no. 4, pp. 249-261.
Wehry, M. ; Penner, J. ; Campbell, F. ; Mullins, Richard ; Rink, R. / Exchange-transfusion with pyridoxalated polymerized hemoglobin : Effects on liver PO2. In: Circulatory Shock. 1987 ; Vol. 23, No. 4. pp. 249-261.
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