Examination of Steroid-Induced Changes in LHRH Gene Transcription Using 33P- and 35S-Labeled Probes Specific for Intron 2

S. L. Petersen, E. Gardner, John Adelman, S. McCrone

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We previously demonstrated that estrogen, but not progesterone, induces an increase in LHRH messenger RNA levels between 0800 and 1200 h in neurons of the rostral preoptic area/organum vasculosum of the lamina terminalis (rPOA/OVLT). To determine whether these changes are a result of increased transcription, we used in situ hybridization histochemistry to examine the temporal effects of estradiol (E2) and progesterone on cellular levels of intron 2-containing LHRH heteronuclear RNA transcripts. To make our method sufficiently sensitive to detect changes in these levels, we used 33P/35S probes to minimize both background and grain stacking over labeled cell nuclei. We found that levels of LHRH heteronuclear RNA rose between 0800 and 1000 in OVX, E2-treated rats, remained elevated at 1200 h, and declined by 1400 h. Progesterone did not affect these E2-induced changes. Our results suggest that an E2-dependent signal initiates LHRH gene transcription in neurons of the rPOA/OVLT hours before LHRH release begins and that progesterone does not affect this signal.

Original languageEnglish (US)
Pages (from-to)234-239
Number of pages6
JournalEndocrinology
Volume137
Issue number1
StatePublished - 1996

Fingerprint

Gonadotropin-Releasing Hormone
Introns
Steroids
Progesterone
Preoptic Area
Genes
RNA
Neurons
Cell Nucleus
In Situ Hybridization
Estradiol
Estrogens
Messenger RNA
Organum Vasculosum

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Examination of Steroid-Induced Changes in LHRH Gene Transcription Using 33P- and 35S-Labeled Probes Specific for Intron 2. / Petersen, S. L.; Gardner, E.; Adelman, John; McCrone, S.

In: Endocrinology, Vol. 137, No. 1, 1996, p. 234-239.

Research output: Contribution to journalArticle

Petersen, SL, Gardner, E, Adelman, J & McCrone, S 1996, 'Examination of Steroid-Induced Changes in LHRH Gene Transcription Using 33P- and 35S-Labeled Probes Specific for Intron 2', Endocrinology, vol. 137, no. 1, pp. 234-239.
Petersen, S. L. ; Gardner, E. ; Adelman, John ; McCrone, S. / Examination of Steroid-Induced Changes in LHRH Gene Transcription Using 33P- and 35S-Labeled Probes Specific for Intron 2. In: Endocrinology. 1996 ; Vol. 137, No. 1. pp. 234-239.
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