Examination of Steroid-Induced Changes in LHRH Gene Transcription Using 33P- and 35S-Labeled Probes Specific for Intron 2

S. L. Petersen, E. Gardner, J. Adelman, S. McCrone

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Abstract

We previously demonstrated that estrogen, but not progesterone, induces an increase in LHRH messenger RNA levels between 0800 and 1200 h in neurons of the rostral preoptic area/organum vasculosum of the lamina terminalis (rPOA/OVLT). To determine whether these changes are a result of increased transcription, we used in situ hybridization histochemistry to examine the temporal effects of estradiol (E2) and progesterone on cellular levels of intron 2-containing LHRH heteronuclear RNA transcripts. To make our method sufficiently sensitive to detect changes in these levels, we used 33P/35S probes to minimize both background and grain stacking over labeled cell nuclei. We found that levels of LHRH heteronuclear RNA rose between 0800 and 1000 in OVX, E2-treated rats, remained elevated at 1200 h, and declined by 1400 h. Progesterone did not affect these E2-induced changes. Our results suggest that an E2-dependent signal initiates LHRH gene transcription in neurons of the rPOA/OVLT hours before LHRH release begins and that progesterone does not affect this signal.

Original languageEnglish (US)
Pages (from-to)234-239
Number of pages6
JournalEndocrinology
Volume137
Issue number1
DOIs
StatePublished - Jan 1 1996

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ASJC Scopus subject areas

  • Endocrinology

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