Ex vivo studies of optic nerve axon electrophysiology

Chinthasagar Bastian, Sylvain Brunet, Selva Baltan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The use of ex vivo compound action potential (CAP) recordings from intact optic nerves is an ideal model to study white matter function without the influence of gray matter. Here, we describe how freshly dissected optic nerves are placed in a humidified recording chamber and how evoked CAPs are recorded and monitored in real time for up to 10 h. Evoked CAP recordings allow for white matter to be studied under acute challenges such as anoxia, hypoxia, aglycemia, and ischemia.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages169-177
Number of pages9
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2143
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Aglycemia
  • Anoxia
  • Axon
  • Compound action potential
  • Ischemia
  • Optic nerve
  • White matter

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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