Ex Vivo selection for oncoretrovirally transduced green fluorescent protein-expressing CD34-enriched cells increases short-term engraftment of transduced cells in baboons

Hans Peter Kiem, John E J Rasko, Julia Morris, Laura Peterson, Peter Kurre, Robert G. Andrews

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In an effort to improve hematopoietic stem cell gene transfer rates using gibbon ape leukemia virus (GALV)-pseudotype retroviral vectors in baboons, we have studied preselection of transduced green fluorescent protein (GFP)-expressing CD34-enriched marrow cells. Three animals were transplanted with GFP-selected (GS) CD34-enriched marrow. To ensure engraftment, preselected GFP-positive cells were infused together with unselected neo-transduced cells. After transduction on fibronectin, cells were cultured for an additional 2 days to allow for expression of GFP. GFP-expressing cells were enriched by fluorescence-activated cell sorting and infused together with cells from the unselected fractions after myeloablative irradiation of the recipient. Three other animals were transplanted with GFP-transduced CD34-enriched cells without prior GFP selection (GU). At 4 weeks after transplant, the percentage of GFP-expressing white blood cells was significantly higher in the GS group (6.6%) than in the GU group (1.3%) (p

Original languageEnglish (US)
Pages (from-to)891-899
Number of pages9
JournalHuman Gene Therapy
Volume13
Issue number8
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Papio
Green Fluorescent Proteins
Gibbon ape leukemia virus
Bone Marrow
Hematopoietic Stem Cells
Fibronectins
Cultured Cells
Flow Cytometry
Leukocytes
Transplants

ASJC Scopus subject areas

  • Genetics

Cite this

Ex Vivo selection for oncoretrovirally transduced green fluorescent protein-expressing CD34-enriched cells increases short-term engraftment of transduced cells in baboons. / Kiem, Hans Peter; Rasko, John E J; Morris, Julia; Peterson, Laura; Kurre, Peter; Andrews, Robert G.

In: Human Gene Therapy, Vol. 13, No. 8, 2002, p. 891-899.

Research output: Contribution to journalArticle

@article{5377d401a96a42c7ba1add438d5247e3,
title = "Ex Vivo selection for oncoretrovirally transduced green fluorescent protein-expressing CD34-enriched cells increases short-term engraftment of transduced cells in baboons",
abstract = "In an effort to improve hematopoietic stem cell gene transfer rates using gibbon ape leukemia virus (GALV)-pseudotype retroviral vectors in baboons, we have studied preselection of transduced green fluorescent protein (GFP)-expressing CD34-enriched marrow cells. Three animals were transplanted with GFP-selected (GS) CD34-enriched marrow. To ensure engraftment, preselected GFP-positive cells were infused together with unselected neo-transduced cells. After transduction on fibronectin, cells were cultured for an additional 2 days to allow for expression of GFP. GFP-expressing cells were enriched by fluorescence-activated cell sorting and infused together with cells from the unselected fractions after myeloablative irradiation of the recipient. Three other animals were transplanted with GFP-transduced CD34-enriched cells without prior GFP selection (GU). At 4 weeks after transplant, the percentage of GFP-expressing white blood cells was significantly higher in the GS group (6.6{\%}) than in the GU group (1.3{\%}) (p",
author = "Kiem, {Hans Peter} and Rasko, {John E J} and Julia Morris and Laura Peterson and Peter Kurre and Andrews, {Robert G.}",
year = "2002",
doi = "10.1089/10430340252939005",
language = "English (US)",
volume = "13",
pages = "891--899",
journal = "Human Gene Therapy",
issn = "1043-0342",
publisher = "Mary Ann Liebert Inc.",
number = "8",

}

TY - JOUR

T1 - Ex Vivo selection for oncoretrovirally transduced green fluorescent protein-expressing CD34-enriched cells increases short-term engraftment of transduced cells in baboons

AU - Kiem, Hans Peter

AU - Rasko, John E J

AU - Morris, Julia

AU - Peterson, Laura

AU - Kurre, Peter

AU - Andrews, Robert G.

PY - 2002

Y1 - 2002

N2 - In an effort to improve hematopoietic stem cell gene transfer rates using gibbon ape leukemia virus (GALV)-pseudotype retroviral vectors in baboons, we have studied preselection of transduced green fluorescent protein (GFP)-expressing CD34-enriched marrow cells. Three animals were transplanted with GFP-selected (GS) CD34-enriched marrow. To ensure engraftment, preselected GFP-positive cells were infused together with unselected neo-transduced cells. After transduction on fibronectin, cells were cultured for an additional 2 days to allow for expression of GFP. GFP-expressing cells were enriched by fluorescence-activated cell sorting and infused together with cells from the unselected fractions after myeloablative irradiation of the recipient. Three other animals were transplanted with GFP-transduced CD34-enriched cells without prior GFP selection (GU). At 4 weeks after transplant, the percentage of GFP-expressing white blood cells was significantly higher in the GS group (6.6%) than in the GU group (1.3%) (p

AB - In an effort to improve hematopoietic stem cell gene transfer rates using gibbon ape leukemia virus (GALV)-pseudotype retroviral vectors in baboons, we have studied preselection of transduced green fluorescent protein (GFP)-expressing CD34-enriched marrow cells. Three animals were transplanted with GFP-selected (GS) CD34-enriched marrow. To ensure engraftment, preselected GFP-positive cells were infused together with unselected neo-transduced cells. After transduction on fibronectin, cells were cultured for an additional 2 days to allow for expression of GFP. GFP-expressing cells were enriched by fluorescence-activated cell sorting and infused together with cells from the unselected fractions after myeloablative irradiation of the recipient. Three other animals were transplanted with GFP-transduced CD34-enriched cells without prior GFP selection (GU). At 4 weeks after transplant, the percentage of GFP-expressing white blood cells was significantly higher in the GS group (6.6%) than in the GU group (1.3%) (p

UR - http://www.scopus.com/inward/record.url?scp=0036000325&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036000325&partnerID=8YFLogxK

U2 - 10.1089/10430340252939005

DO - 10.1089/10430340252939005

M3 - Article

C2 - 12031122

AN - SCOPUS:0036000325

VL - 13

SP - 891

EP - 899

JO - Human Gene Therapy

JF - Human Gene Therapy

SN - 1043-0342

IS - 8

ER -