Abstract
The evolutionary history of human chromosome 20 in primates was investigated using a panel of human BAC/PAC probes spaced along the chromosome. Oligonucleotide primers derived from the sequence of each human clone were used to screen horse, cat, pig, and black lemur BAC libraries to assemble, for each species, a panel of probes mapping to chromosomal loci orthologous to the loci encompassed by the human BACs. This approach facilitated marker-order comparison aimed at defining marker arrangement in primate ancestor. To this goal, we also took advantage of the mouse and rat draft sequences. The almost perfect colinearity of chromosome 20 sequence in humans and mouse could be interpreted as evidence that their form was ancestral to primates. Contrary to this view, we found that horse, macaque, and two New World monkeys share the same marker-order arrangement from which the human and mouse forms can be derived, assuming similar but distinct inversions that fully account for the small difference in marker arrangement between humans and mouse. The evolutionary history of this chromosome unveiled also two centromere repositioning events in New World monkey species.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 360-366 |
| Number of pages | 7 |
| Journal | Molecular Biology and Evolution |
| Volume | 22 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2005 |
| Externally published | Yes |
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Keywords
- Chromosome 20
- Chromosome evolution
- Neocentromeres
ASJC Scopus subject areas
- Genetics
- Biochemistry
- Genetics(clinical)
- Biochemistry, Genetics and Molecular Biology(all)
- Ecology, Evolution, Behavior and Systematics
- Agricultural and Biological Sciences (miscellaneous)
- Molecular Biology
Cite this
Evolutionary history of chromosome 20. / Misceo, Doriana; Cardone, Maria Francesca; Carbone, Lucia; D'Addabbo, Pietro; De Jong, Pieter J.; Rocchi, Mariano; Archidiacono, Nicoletta.
In: Molecular Biology and Evolution, Vol. 22, No. 2, 02.2005, p. 360-366.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evolutionary history of chromosome 20
AU - Misceo, Doriana
AU - Cardone, Maria Francesca
AU - Carbone, Lucia
AU - D'Addabbo, Pietro
AU - De Jong, Pieter J.
AU - Rocchi, Mariano
AU - Archidiacono, Nicoletta
PY - 2005/2
Y1 - 2005/2
N2 - The evolutionary history of human chromosome 20 in primates was investigated using a panel of human BAC/PAC probes spaced along the chromosome. Oligonucleotide primers derived from the sequence of each human clone were used to screen horse, cat, pig, and black lemur BAC libraries to assemble, for each species, a panel of probes mapping to chromosomal loci orthologous to the loci encompassed by the human BACs. This approach facilitated marker-order comparison aimed at defining marker arrangement in primate ancestor. To this goal, we also took advantage of the mouse and rat draft sequences. The almost perfect colinearity of chromosome 20 sequence in humans and mouse could be interpreted as evidence that their form was ancestral to primates. Contrary to this view, we found that horse, macaque, and two New World monkeys share the same marker-order arrangement from which the human and mouse forms can be derived, assuming similar but distinct inversions that fully account for the small difference in marker arrangement between humans and mouse. The evolutionary history of this chromosome unveiled also two centromere repositioning events in New World monkey species.
AB - The evolutionary history of human chromosome 20 in primates was investigated using a panel of human BAC/PAC probes spaced along the chromosome. Oligonucleotide primers derived from the sequence of each human clone were used to screen horse, cat, pig, and black lemur BAC libraries to assemble, for each species, a panel of probes mapping to chromosomal loci orthologous to the loci encompassed by the human BACs. This approach facilitated marker-order comparison aimed at defining marker arrangement in primate ancestor. To this goal, we also took advantage of the mouse and rat draft sequences. The almost perfect colinearity of chromosome 20 sequence in humans and mouse could be interpreted as evidence that their form was ancestral to primates. Contrary to this view, we found that horse, macaque, and two New World monkeys share the same marker-order arrangement from which the human and mouse forms can be derived, assuming similar but distinct inversions that fully account for the small difference in marker arrangement between humans and mouse. The evolutionary history of this chromosome unveiled also two centromere repositioning events in New World monkey species.
KW - Chromosome 20
KW - Chromosome evolution
KW - Neocentromeres
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UR - http://www.scopus.com/inward/citedby.url?scp=12344252043&partnerID=8YFLogxK
U2 - 10.1093/molbev/msi021
DO - 10.1093/molbev/msi021
M3 - Article
C2 - 15496555
AN - SCOPUS:12344252043
VL - 22
SP - 360
EP - 366
JO - Molecular Biology and Evolution
JF - Molecular Biology and Evolution
SN - 0737-4038
IS - 2
ER -