Evolutionary conservation of tissue-specific lymphocyte-endothelial cell recognition mechanisms involved in lymphocyte homing

N. W. Wu, S. Jalkanen, Philip Streeter, E. C. Butcher

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Tissue-specific interactions with specialized high endothelial venules (HEV) direct the homing of lymphocytes from the blood into peripheral lymph nodes, mucosal lymphoid organs, and tissue sites of chronic inflammation. These interactions have been demonstrated in all mammalian species examined and thus appear highly conserved. To assess the degree of evolutionary divergence in lymphocyte-HEV recognition mechanisms, we have studied the ability of lymphocytes to interact with HEV across species barriers. By using an in vitro assay of lymphocyte binding to HEV in frozen sections of lymphoid tissues, we confirm that mouse, guinea pig, and human lymphocytes bind to xenogeneic as well as homologous HEV. In addition, we show that mouse and human lymphoid cell lines that bind selectively to either peripheral lymph node or mucosal vessels (Peyer's patches, appendix) in homologous lymphoid tissues exhibit the same organ specificity in binding to xenogeneic HEV. Furthermore, monoclonal antibodies that recognize lymphocyte 'homing receptors' and block homologous lymphocyte binding to peripheral lymph node or to mucosal HEV, also inhibit lymphocyte interactions with xenogeneic HEV in a tissue-specific fashion. Similarly, anti-HEV antibodies against organ-specific mouse high endothelial cell 'addressins' involved in lymphocyte homing to peripheral lymph node or mucosal lymphoid organs, not only block the adhesion of mouse lymphocytes but also of human lymphocytes to target mouse HEV. The results illustrate a remarkable degree of functional conservation of elements mediating these cell-cell recognition events involved in organ-specific lymphocyte homing.

Original languageEnglish (US)
Pages (from-to)1845-1851
Number of pages7
JournalJournal of Cell Biology
Volume107
Issue number5
StatePublished - 1988
Externally publishedYes

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Venules
Endothelial Cells
Lymphocytes
Lymphoid Tissue
Lymph Nodes
Lymphocyte Homing Receptors
Peyer's Patches
Organ Specificity
Frozen Sections
Guinea Pigs
Monoclonal Antibodies
Inflammation
Cell Line

ASJC Scopus subject areas

  • Cell Biology

Cite this

Evolutionary conservation of tissue-specific lymphocyte-endothelial cell recognition mechanisms involved in lymphocyte homing. / Wu, N. W.; Jalkanen, S.; Streeter, Philip; Butcher, E. C.

In: Journal of Cell Biology, Vol. 107, No. 5, 1988, p. 1845-1851.

Research output: Contribution to journalArticle

Wu, N. W. ; Jalkanen, S. ; Streeter, Philip ; Butcher, E. C. / Evolutionary conservation of tissue-specific lymphocyte-endothelial cell recognition mechanisms involved in lymphocyte homing. In: Journal of Cell Biology. 1988 ; Vol. 107, No. 5. pp. 1845-1851.
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