Evolution of insulin-like growth factor II: Characterization of the mouse IGF-II gene and identification of two pseudo-exons

P. Rotwein, L. J. Hall

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

We have cloned the mouse insulin-like growth factor II (IGF-II) gene as a series of overlapping cosmid and λ recombinants and have characterized its six exons. The gene extends over approximately 12 kb of mouse chromosome 7 and is located 18 kb 3' to the insulin 2 gene and in the same transcriptional polarity. Exons 1-3 encode distinct 5' untranslated regions and are transcribed by three different promoters, P1, P2, and P3, into three IGF-II mRNAs sharing common coding and 3' untranslated sequences. Promoters P2 and P3 each contain a TATA box and appear to direct transcription from single initiation sites. By contrast, exon 1 has three major transcriptional start sites distributed over 556 nucleotides, and P1 lacks a TATA region and other typical transcriptional control sequences. Exons 4-6 code for the 180-amino-acid IGF-II precursor, and exon 6 also contains a 3,045-nucleotide 3' untranslated region which ends at a single polyadenylation site. In addition to six functional IGF-II exons, we identified two 5' 'pseudo-exons,' which appear to be evolutionarily retained remnants of an alternative promoter-exon cassette that is active in human IGF-II. Loss of the homolog of this promoter, which directs 'adult-specific' expression of the IGF-II gene in some human tissues, may explain the disappearance of this growth factor from most murine tissues in the early postnatal period.

Original languageEnglish (US)
Pages (from-to)725-735
Number of pages11
JournalDNA and Cell Biology
Volume9
Issue number10
StatePublished - 1990
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor II
Exons
Genes
Nucleotides
Cosmids
TATA Box
Polyadenylation
Chromosomes, Human, Pair 7
5' Untranslated Regions
Transcription Initiation Site
3' Untranslated Regions
Intercellular Signaling Peptides and Proteins
Insulin
Amino Acids
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Evolution of insulin-like growth factor II : Characterization of the mouse IGF-II gene and identification of two pseudo-exons. / Rotwein, P.; Hall, L. J.

In: DNA and Cell Biology, Vol. 9, No. 10, 1990, p. 725-735.

Research output: Contribution to journalArticle

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