Evidence that the Rhodopsin Kinase (GRK1) N-Terminus and the Transducin Gα C-Terminus Interact with the Same "hydrophobic Patch" on Rhodopsin TM5

Amber M. Jones Brunette, Abhinav Sinha, Larry David, David Farrens

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Phosphorylation of G protein-coupled receptors (GPCRs) terminates their ability to couple with and activate G proteins by increasing their affinity for arrestins. Unfortunately, detailed information regarding how GPCRs interact with the kinases responsible for their phosphorylation is still limited. Here, we purified fully functional GPCR kinase 1 (GRK1) using a rapid method and used it to gain insights into how this important kinase interacts with the GPCR rhodopsin. Specifically, we find that GRK1 uses the same site on rhodopsin as the transducin (Gt) G C-terminal tail and the arrestin "finger loop", a cleft formed in the cytoplasmic face of the receptor upon activation. Our studies also show GRK1 requires two conserved residues located in this cleft (L226 and V230) that have been shown to be required for Gt activation due to their direct interactions with hydrophobic residues on the Gα C-terminal tail. Our data and modeling studies are consistent with the idea that all three proteins (Gt, GRK1, and visual arrestin) bind, at least in part, in the same site on rhodopsin and interact with the receptor through a similar hydrophobic contact-driven mechanism.

Original languageEnglish (US)
Pages (from-to)3123-3135
Number of pages13
JournalBiochemistry
Volume55
Issue number22
DOIs
StatePublished - Jun 7 2016

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G-Protein-Coupled Receptor Kinase 1
Transducin
Rhodopsin
Phosphotransferases
G-Protein-Coupled Receptors
Arrestin
Phosphorylation
Chemical activation
Arrestins
Cytoplasmic and Nuclear Receptors
Hydrophobic and Hydrophilic Interactions
GTP-Binding Proteins
Fingers
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Evidence that the Rhodopsin Kinase (GRK1) N-Terminus and the Transducin Gα C-Terminus Interact with the Same "hydrophobic Patch" on Rhodopsin TM5. / Jones Brunette, Amber M.; Sinha, Abhinav; David, Larry; Farrens, David.

In: Biochemistry, Vol. 55, No. 22, 07.06.2016, p. 3123-3135.

Research output: Contribution to journalArticle

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