Evidence that the modulatory effect of galanin on inflammatory edema formation is mediated by the galanin receptor 3 in the murine microvasculature

Sabine M. Schmidhuber, Isabella Rauch, Barbara Kofler, Susan D. Brain

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Neuropeptides released from cutaneous nerves are attracting interest as modulators of inflammation in the skin. Recently, we showed that the neuropeptides galanin and galanin-like peptide potently inhibit inflammatory edema by reduction of microvascular blood flow. Reverse transcription-polymerase chain reaction analysis of murine skin revealed the expression of galanin receptors 2 and 3. The aim of the present study was to elucidate which galanin receptor subtype mediates the galanin-evoked inhibition of inflammatory edema formation in the skin. In this study, we report that AR-M1896, a non-GalR1 agonist, inhibited plasma extravasation induced by substance P and calcitonin gene-related peptide in a manner similar to galanin, confirming a non-GalR1-mediated effect. SNAP 37889, a nonpeptidergic selective antagonist of galanin receptor 3 (GalR3), dose-dependently abolished the antiedema effect of galanin. Thus, we were able to show that SNAP 37889 selectively antagonized galanin in the periphery and suggest that GalR3 is the receptor subtype mediating galanin's effects on the dermal microvasculature.

Original languageEnglish (US)
Pages (from-to)177-181
Number of pages5
JournalJournal of Molecular Neuroscience
Volume37
Issue number2
DOIs
StatePublished - Feb 1 2009

Keywords

  • Edema
  • Galanin
  • Microvasculature
  • Mouse skin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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