Evidence that synthetic 31-amino acid inhibin-like peptide lacks inhibin activity

L. Liu, J. Booth, G. R. Merriam, K. M. Barnes, R. J. Sherins, Donald (Lynn) Loriaux, G. B. Cutler

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We studied the biological activity of a recently characterized 31-amino acid inhibin-like peptide (ILP) using a synthetic preparation. While the material yielded a single component on chromatography, amino acid sequence analysis suggested that only 30% of the molecules possess the complete structure. Bioactivity was tested in vitro using whole pituitaries from 25 day-old male Sprague-Dawley rats. Pituitaries were incubated with 500 ng/ml of the ILP preparation or vehicle alone for 60 min, followed by a 3 h exposure to 2 ng/ml of luteinizing hormone releasing hormone (LHRH) or its diluent. In the ILP-incubated pituitary media, no significant suppression of basal FSH and LH release or stimulated FSH release was observed, while a significant increase in stimulated LH release was seen (p

Original languageEnglish (US)
Pages (from-to)191-197
Number of pages7
JournalEndocrine Research
Volume11
Issue number3-4
StatePublished - 1985
Externally publishedYes

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Inhibins
Amino Acids
Protein Sequence Analysis
Gonadotropin-Releasing Hormone
Sprague Dawley Rats
Chromatography
human inhibin-like peptide (1-31)

ASJC Scopus subject areas

  • Endocrinology

Cite this

Liu, L., Booth, J., Merriam, G. R., Barnes, K. M., Sherins, R. J., Loriaux, D. L., & Cutler, G. B. (1985). Evidence that synthetic 31-amino acid inhibin-like peptide lacks inhibin activity. Endocrine Research, 11(3-4), 191-197.

Evidence that synthetic 31-amino acid inhibin-like peptide lacks inhibin activity. / Liu, L.; Booth, J.; Merriam, G. R.; Barnes, K. M.; Sherins, R. J.; Loriaux, Donald (Lynn); Cutler, G. B.

In: Endocrine Research, Vol. 11, No. 3-4, 1985, p. 191-197.

Research output: Contribution to journalArticle

Liu, L, Booth, J, Merriam, GR, Barnes, KM, Sherins, RJ, Loriaux, DL & Cutler, GB 1985, 'Evidence that synthetic 31-amino acid inhibin-like peptide lacks inhibin activity', Endocrine Research, vol. 11, no. 3-4, pp. 191-197.
Liu L, Booth J, Merriam GR, Barnes KM, Sherins RJ, Loriaux DL et al. Evidence that synthetic 31-amino acid inhibin-like peptide lacks inhibin activity. Endocrine Research. 1985;11(3-4):191-197.
Liu, L. ; Booth, J. ; Merriam, G. R. ; Barnes, K. M. ; Sherins, R. J. ; Loriaux, Donald (Lynn) ; Cutler, G. B. / Evidence that synthetic 31-amino acid inhibin-like peptide lacks inhibin activity. In: Endocrine Research. 1985 ; Vol. 11, No. 3-4. pp. 191-197.
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