TY - JOUR
T1 - Evidence that clonal anergy is induced in thymic migrant cells after anti-cd4-mediated transplantation tolerance
AU - Alters, Susan E.
AU - Song, Howard K.
AU - Fathman, C. Garrison
PY - 1993/9
Y1 - 1993/9
N2 - Diabetic (B6) (IE“) mice treated with a depleting regimen of anti-CD4 monoclonal antibody at the time of transplantation with A/J (IEk) islets of Langerhans showed indefinite acceptance of their islet allograft, as evidenced by persistent normoglycemia. To address the mechanisms involved in such anti-CD4 induced transplantation tolerance we studied potentially IE-reactive Vβ11+ T cells from the tolerant allografted mice. Following complete repopulation of the CD4+ cells, both the CD4+Vβ11+ and CD8+11+ T cell subsets of the transplanted mice were unresponsive to anti-Vβ11 specific crosslinking. In contrast, lymphocytes tested within the first ten days following transplant were responsive to anti-Vβ11 specific crosslinking; this response decreased as a function of time and reached background levels by day 120 posttransplant. Sorting experiments indicated that the response of lymphocytes to anti-Vβ11 specific crosslinking seen during the initial 120 days posttransplant was confined to the peripheral CD8+ cells; the repopulating CD4+Vβ11+ T cells were unresponsive. In addition, administration of r-IL-2 at the time of transplantation induced rejection in anti-CD4-treated animals, again indicating that the peripheral CD8+ cells could respond shortly after transplant if provided with appropriate help. The decreasing response of CD8+ T cells from transplanted animals to anti-Vβ11 stimulation was inversely correlated with the rate of migration of cells from the thymus to the periphery, implying that new thymic migrant Vβ11+ cells, both CD4+ and CD8+, were rendered anergic upon encountering peripheral alloantigen. These data suggest the possibility that recent thymic migrants are rendered anergic upon encountering antigen in the periphery, a simple model to serve as a “fail-safe” mechanism to prevent autoreactivity.
AB - Diabetic (B6) (IE“) mice treated with a depleting regimen of anti-CD4 monoclonal antibody at the time of transplantation with A/J (IEk) islets of Langerhans showed indefinite acceptance of their islet allograft, as evidenced by persistent normoglycemia. To address the mechanisms involved in such anti-CD4 induced transplantation tolerance we studied potentially IE-reactive Vβ11+ T cells from the tolerant allografted mice. Following complete repopulation of the CD4+ cells, both the CD4+Vβ11+ and CD8+11+ T cell subsets of the transplanted mice were unresponsive to anti-Vβ11 specific crosslinking. In contrast, lymphocytes tested within the first ten days following transplant were responsive to anti-Vβ11 specific crosslinking; this response decreased as a function of time and reached background levels by day 120 posttransplant. Sorting experiments indicated that the response of lymphocytes to anti-Vβ11 specific crosslinking seen during the initial 120 days posttransplant was confined to the peripheral CD8+ cells; the repopulating CD4+Vβ11+ T cells were unresponsive. In addition, administration of r-IL-2 at the time of transplantation induced rejection in anti-CD4-treated animals, again indicating that the peripheral CD8+ cells could respond shortly after transplant if provided with appropriate help. The decreasing response of CD8+ T cells from transplanted animals to anti-Vβ11 stimulation was inversely correlated with the rate of migration of cells from the thymus to the periphery, implying that new thymic migrant Vβ11+ cells, both CD4+ and CD8+, were rendered anergic upon encountering peripheral alloantigen. These data suggest the possibility that recent thymic migrants are rendered anergic upon encountering antigen in the periphery, a simple model to serve as a “fail-safe” mechanism to prevent autoreactivity.
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U2 - 10.1097/00007890-199309000-00026
DO - 10.1097/00007890-199309000-00026
M3 - Article
C2 - 8212161
AN - SCOPUS:0027435680
SN - 0041-1337
VL - 56
SP - 633
EP - 638
JO - Transplantation
JF - Transplantation
IS - 3
ER -