Evidence for translational regulation of herpes simplex virus type 1 gD expression

David Johnson, P. G. Spear

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We compared the rates of synthesis of herpes simplex virus type 1 glycoproteins C and D and quantitated the accumulation of translatable mRNA for each glycoprotein at various times after infection. The rate of synthesis of gD increased sharply early in the infection, peaked by 4 to 6 hr after infection, and declined late in the infection. In contrast, the rate of synthesis of gC increased steadily until at least 15 h after infection. The levels of mRNA for both of these glycoproteins, as detected by hybridization and by translation in vitro, continued to increase until at least 15 or 16 hr after infection. Synthesis of both gC and gD and their respective mRNAs was found to be sensitive to inhibition of viral DNA replication with phosphonoacetic acid. The finding that reduced amounts of gD were synthesized late in the replicative cycle, whereas gD mRNA continued to accumulate in the cytoplasm, argues that the synthesis of gD is regulated, in part, at the level of translation.

Original languageEnglish (US)
Pages (from-to)389-394
Number of pages6
JournalJournal of Virology
Volume51
Issue number2
StatePublished - 1984
Externally publishedYes

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Human herpesvirus 1
Human Herpesvirus 1
Infection
synthesis
infection
glycoproteins
Messenger RNA
translation (genetics)
Glycoproteins
Phosphonoacetic Acid
Viral DNA
DNA replication
DNA Replication
Cytoplasm
hybridization
cytoplasm
acids

ASJC Scopus subject areas

  • Immunology

Cite this

Evidence for translational regulation of herpes simplex virus type 1 gD expression. / Johnson, David; Spear, P. G.

In: Journal of Virology, Vol. 51, No. 2, 1984, p. 389-394.

Research output: Contribution to journalArticle

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