Evidence for the lack of mismatch-repair directed antirecombination during mouse meiosis

J. Qin, S. Baker, H. Te Riele, R. M. Liskay, Norman Arnheim

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Abstract

Meiotic recombination was studied in DNA mismatch repair (MMR)-deficient mice using a strain carrying a Pms2 knockout mutation. Using single-sperm typing, recombination was analyzed over five intervals on four chromosomes in four Pms2 -/- animals. A total of 1936 meioses were studied and compared to 1848 meioses from three Pms2 +/+ controls. A smaller study was carried out on a single interval in each of two chromosomes in an MMR-deficient mouse homozygous for the Msh2 knockout mutation. A total of 792 meioses were examined in the Msh2 -/- and 880 meioses in the Msh2 +/+ animal. Recombination fractions were not significantly different in either of the MMR-deficient mouse strains when compared to MMR-proficient controls. Our results appear to conflict with mouse embryonic stem (ES) cell gene-targeting experiments where MMR plays a major role in determining the efficiency of homologous recombination between nonidentical sequences. A number of possibilities could explain the apparent lack of a significant effect on meiosis.

Original languageEnglish (US)
Pages (from-to)201-205
Number of pages5
JournalJournal of Heredity
Volume93
Issue number3
StatePublished - May 1 2002

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ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Qin, J., Baker, S., Te Riele, H., Liskay, R. M., & Arnheim, N. (2002). Evidence for the lack of mismatch-repair directed antirecombination during mouse meiosis. Journal of Heredity, 93(3), 201-205.