Evidence for the existence of substance P in the prepubertal rat ovary. I. Biochemical and physiologic studies

Sergio Ojeda, M. E. Costa, K. H. Katz, L. B. Hersh

Research output: Contribution to journalArticle

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Abstract

The presence of substance P (SP) in the immature rat ovary was determined by radioimmunoassay (RIA) of acidic extracts. The extracts produced an inhibition-displacement curve of 125I-SP binding parallel to that generated by authentic SP in the SP RIA. Initial chromatography characterization of ovarian SP in Sephadex G-25 revealed the presence of a molecular form that coeluted with authentic SP and a more abundant component that eluted earlier, suggesting the presence of a heavier peptide, immunologically similar to SP. Nevertheless, further characterization of these two seemingly different components by reversed-phase high-performance liquid chromatography (HPLC) demonstrated that both of them had a retention time similar to that of authentic SP. The ovarian concentration of SP-like immunoreactivity (SP-LI) varied in relation to the onset of puberty, with values increasing significantly between the late juvenile phase and the day of first proestrus. Substance P seems to be devoid of steroidogenic capacity since SP itself and its stable analog [pGlu5, MePhe8,Sar9]-SP5-11 (SP-A) failed to stimulate steroid secretion from either granulosa cells in culture or ovarian fragments in short-term incubation. Substance P also failed to stimulate prostaglandin E2 release from whole ovaries and to modify the steroidal response of cultured granulosa cells to follicle hormone and to the β2-adrenergic agonist Zinterol. Production of SP-LI from granulosa cells in culture could not be detected under either basal or gonadotropin-stimulated conditions. These observations and the distribution of the peptide within the ovary presented in the companion paper (Dees et al., this issue) strongly suggest that SP is not directly involved in regulating steroidogenesis. Instead, SP may be a component of the so-called sensory innervation of the ovary, and among other undisclosed functions it may contribute to the regulation of ovarian blood flow.

Original languageEnglish (US)
Pages (from-to)286-295
Number of pages10
JournalBiology of Reproduction
Volume33
Issue number2
StatePublished - 1985
Externally publishedYes

Fingerprint

Substance P
Ovary
Granulosa Cells
Radioimmunoassay
Cell Culture Techniques
Proestrus
Adrenergic Agonists
Peptides
Reverse-Phase Chromatography
Puberty
Gonadotropins
Dinoprostone

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Evidence for the existence of substance P in the prepubertal rat ovary. I. Biochemical and physiologic studies. / Ojeda, Sergio; Costa, M. E.; Katz, K. H.; Hersh, L. B.

In: Biology of Reproduction, Vol. 33, No. 2, 1985, p. 286-295.

Research output: Contribution to journalArticle

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