Evidence for superantigen involvement in skin homing of T cells in atopic dermatitis

Ian Strickland, Pia J. Hauk, Anne E. Trumble, Louis Picker, Donald Y M Leung

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The environmental factors that contribute to the homing of T cells in skin disease is unknown. The skin lesions of atopic dermatitis (AD) are frequently colonized with superantigen (SAg), producing strains of Staphylococcus aureus. In vitro, these superantigens have the capacity to activate and expand T cells expressing specific T cell receptor BV gene segments, and also to increase their skin homing capacity via upregulation of the skin homing receptor, cutaneous lymphocyte-associated antigen (CLA). These activities have been proposed to enhance the chronic cutaneous inflammation of AD, but an in vivo role for SAg has not been conclusively demonstrated. In this study, we sought direct evidence for in vivo SAg activity by comparing the SAg profiles of S. aureus cultured from the skin of AD subjects to the T cell receptor Vβ repertoire of their skin homing (CLA+) T cells in peripheral blood. SAg secreting S. aureus strains were identified in six of 12 AD patients, and all of these subjects manifested significant SAg-appropriate Vβ skewing within the CLA+ subsets of both their CD4+ and their CD8+ T cells. T cell receptor Vβ skewing was not detectable among the overall CD4+ or CD8+ T cell subsets of these subjects, and was not present within the CLA+ T cell subsets of five patients with plaque psoriasis and 10 normal controls. T cell receptor BV genes from the presumptively SAg-expanded populations of skin homing T cells were cloned and sequenced from three subjects and, consistent with a SAg-driven effect, were found to be polyclonal. We conclude that SAg can contribute to AD pathogenesis by increasing the frequency of memory T cells able to migrate to and be activated within AD lesions.

Original languageEnglish (US)
Pages (from-to)249-253
Number of pages5
JournalJournal of Investigative Dermatology
Volume112
Issue number2
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Superantigens
T-cells
Atopic Dermatitis
Skin
T-Lymphocytes
Lymphocytes
T-Cell Antigen Receptor
Staphylococcus aureus
T-Cell Receptor Genes
Antigens
T-Lymphocyte Subsets
Genes
Lymphocyte Homing Receptors
Psoriasis
Skin Diseases
Blood
Up-Regulation
Data storage equipment

Keywords

  • CLA
  • Inflammation
  • SEB
  • TSST-1

ASJC Scopus subject areas

  • Dermatology

Cite this

Evidence for superantigen involvement in skin homing of T cells in atopic dermatitis. / Strickland, Ian; Hauk, Pia J.; Trumble, Anne E.; Picker, Louis; Leung, Donald Y M.

In: Journal of Investigative Dermatology, Vol. 112, No. 2, 1999, p. 249-253.

Research output: Contribution to journalArticle

Strickland, Ian ; Hauk, Pia J. ; Trumble, Anne E. ; Picker, Louis ; Leung, Donald Y M. / Evidence for superantigen involvement in skin homing of T cells in atopic dermatitis. In: Journal of Investigative Dermatology. 1999 ; Vol. 112, No. 2. pp. 249-253.
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