Abstract
Purpose. To study the role of anti-recoverin antibodies in cancer associated retinopathy (CAR). Recoverin was identified as an autoantigen in CAR and anti-recoverin antibodies were found in the sera of some CAR patients. Methods. Human, rat, or rabbit antibodies against recoverin were purified using a recoverin-affinity column. Titer and specificity of antibodies were measured by ELISA. Normal human, rat, and rabbit IgG were used in control experiments. Purified antibodies were cultured with a recoverin positive rat retinal cell line EIA-NR3 (105 cells/ml). Antibody uptake in vitro was analyzed by immunocytochemistry. Cytotoxic effect of antibodies on retinal cells was measured by MTT colorimetric method. Results. Our data demonstrated that anti-recoverin antibodies obtained from a CAR patient's serum or from sera of immunized animals were taken up by photoreceptor cells. Interestingly, all antibodies showed similar fine specificities to recoverin in a pin ELISA test. Moreover, these antibodies produced destruction of the cells in a dose and time-dependent manner. Normal IgG did not have such affects on retinal cells. Conclusions. Our studies showed that antibodies specific to recoverin are able to enter and cause death of cells expressing recoverin. In humans, the autoantibodies originally elicited against recoverin expressed in tumor cells may damage the retinal photoreceptors and play a role in pathogenesis of CAR.
| Original language | English (US) |
|---|---|
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 37 |
| Issue number | 3 |
| State | Published - Feb 15 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Ophthalmology
Cite this
Evidence for pathogenic effect of anti-recoverin antibodies in Cancer Associated Retinopathy (CAR). In vitro study. / Machnick, M.; Seigel, G. M.; Adamus, Grazyna.
In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evidence for pathogenic effect of anti-recoverin antibodies in Cancer Associated Retinopathy (CAR). In vitro study
AU - Machnick, M.
AU - Seigel, G. M.
AU - Adamus, Grazyna
PY - 1996/2/15
Y1 - 1996/2/15
N2 - Purpose. To study the role of anti-recoverin antibodies in cancer associated retinopathy (CAR). Recoverin was identified as an autoantigen in CAR and anti-recoverin antibodies were found in the sera of some CAR patients. Methods. Human, rat, or rabbit antibodies against recoverin were purified using a recoverin-affinity column. Titer and specificity of antibodies were measured by ELISA. Normal human, rat, and rabbit IgG were used in control experiments. Purified antibodies were cultured with a recoverin positive rat retinal cell line EIA-NR3 (105 cells/ml). Antibody uptake in vitro was analyzed by immunocytochemistry. Cytotoxic effect of antibodies on retinal cells was measured by MTT colorimetric method. Results. Our data demonstrated that anti-recoverin antibodies obtained from a CAR patient's serum or from sera of immunized animals were taken up by photoreceptor cells. Interestingly, all antibodies showed similar fine specificities to recoverin in a pin ELISA test. Moreover, these antibodies produced destruction of the cells in a dose and time-dependent manner. Normal IgG did not have such affects on retinal cells. Conclusions. Our studies showed that antibodies specific to recoverin are able to enter and cause death of cells expressing recoverin. In humans, the autoantibodies originally elicited against recoverin expressed in tumor cells may damage the retinal photoreceptors and play a role in pathogenesis of CAR.
AB - Purpose. To study the role of anti-recoverin antibodies in cancer associated retinopathy (CAR). Recoverin was identified as an autoantigen in CAR and anti-recoverin antibodies were found in the sera of some CAR patients. Methods. Human, rat, or rabbit antibodies against recoverin were purified using a recoverin-affinity column. Titer and specificity of antibodies were measured by ELISA. Normal human, rat, and rabbit IgG were used in control experiments. Purified antibodies were cultured with a recoverin positive rat retinal cell line EIA-NR3 (105 cells/ml). Antibody uptake in vitro was analyzed by immunocytochemistry. Cytotoxic effect of antibodies on retinal cells was measured by MTT colorimetric method. Results. Our data demonstrated that anti-recoverin antibodies obtained from a CAR patient's serum or from sera of immunized animals were taken up by photoreceptor cells. Interestingly, all antibodies showed similar fine specificities to recoverin in a pin ELISA test. Moreover, these antibodies produced destruction of the cells in a dose and time-dependent manner. Normal IgG did not have such affects on retinal cells. Conclusions. Our studies showed that antibodies specific to recoverin are able to enter and cause death of cells expressing recoverin. In humans, the autoantibodies originally elicited against recoverin expressed in tumor cells may damage the retinal photoreceptors and play a role in pathogenesis of CAR.
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M3 - Article
AN - SCOPUS:33750165205
VL - 37
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
SN - 0146-0404
IS - 3
ER -