Evidence for non-V3-specific neutralizing antibodies that interfere with gp120/CD4 binding in human immunodeficiency virus 1-infected humans

Chang Yuil Kang, Peter Nara, Soulaima Chamat, Vince Caralli, Tom Ryskamp, Nancy Haigwood, Roland Newman, Heinz Köhler

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Total anti-gp120 antibodies (total anti-gp120 Abs) were purified from a pool of four human immunodeficiency virus-positive (HIV+) sera by affinity chromatography on a gp120SF2-Sepharose column and exhibited both type- and group-specific neutralizing activities. To dissect the epitope specificity of the group-specific neutralizing antibodies, CD4 attachment site-specific antibodies (CD4-site Abs) were isolated from total anti-gp120 Abs by using a CD4-blocked gp120SF2-Sepharose column. The CD4-site Abs exhibited group-specific neutralizing activities. Another approach to dissecting type-and group-specific neutralizing activities of total anti-gp120 Abs was to separate the third variable region (V3)-specific antibodies (V3-region Abs) from non-V3-region-specific antibodies (non-V3 Abs). The results indicated that V3-region Abs exhibited type-specific neutralizing activities, whereas non-V3 Abs exhibited group-specific neutralizing activities. By comparing the neutralizing activities of V3-region Abs to those of non-V3 Abs, we concluded that V3-region Abs are more effective than non-V3 Abs in neutralizing a specific HIV isolate. Collectively, this study indicates that group-specific neutralizing anti-gp120 antibodies are specific for the CD4 attachment site.

Original languageEnglish (US)
Pages (from-to)6171-6175
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number14
StatePublished - Jul 15 1991
Externally publishedYes

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Neutralizing Antibodies
HIV-1
Antibodies
HIV
Sepharose
Anti-Idiotypic Antibodies
Affinity Chromatography
Epitopes
Serum

Keywords

  • Human anti-gp120 antibody
  • Human immunodeficiency virus 1 neutralization

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Evidence for non-V3-specific neutralizing antibodies that interfere with gp120/CD4 binding in human immunodeficiency virus 1-infected humans. / Kang, Chang Yuil; Nara, Peter; Chamat, Soulaima; Caralli, Vince; Ryskamp, Tom; Haigwood, Nancy; Newman, Roland; Köhler, Heinz.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 14, 15.07.1991, p. 6171-6175.

Research output: Contribution to journalArticle

Kang, Chang Yuil ; Nara, Peter ; Chamat, Soulaima ; Caralli, Vince ; Ryskamp, Tom ; Haigwood, Nancy ; Newman, Roland ; Köhler, Heinz. / Evidence for non-V3-specific neutralizing antibodies that interfere with gp120/CD4 binding in human immunodeficiency virus 1-infected humans. In: Proceedings of the National Academy of Sciences of the United States of America. 1991 ; Vol. 88, No. 14. pp. 6171-6175.
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