Evidence for non-V3-specific neutralizing antibodies that interfere with gp120/CD4 binding in human immunodeficiency virus 1-infected humans

Chang Yuil Kang, Peter Nara, Soulaima Chamat, Vince Caralli, Tom Ryskamp, Nancy Haigwood, Roland Newman, Heinz Köhler

    Research output: Contribution to journalArticle

    72 Scopus citations

    Abstract

    Total anti-gp120 antibodies (total anti-gp120 Abs) were purified from a pool of four human immunodeficiency virus-positive (HIV+) sera by affinity chromatography on a gp120SF2-Sepharose column and exhibited both type- and group-specific neutralizing activities. To dissect the epitope specificity of the group-specific neutralizing antibodies, CD4 attachment site-specific antibodies (CD4-site Abs) were isolated from total anti-gp120 Abs by using a CD4-blocked gp120SF2-Sepharose column. The CD4-site Abs exhibited group-specific neutralizing activities. Another approach to dissecting type-and group-specific neutralizing activities of total anti-gp120 Abs was to separate the third variable region (V3)-specific antibodies (V3-region Abs) from non-V3-region-specific antibodies (non-V3 Abs). The results indicated that V3-region Abs exhibited type-specific neutralizing activities, whereas non-V3 Abs exhibited group-specific neutralizing activities. By comparing the neutralizing activities of V3-region Abs to those of non-V3 Abs, we concluded that V3-region Abs are more effective than non-V3 Abs in neutralizing a specific HIV isolate. Collectively, this study indicates that group-specific neutralizing anti-gp120 antibodies are specific for the CD4 attachment site.

    Original languageEnglish (US)
    Pages (from-to)6171-6175
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume88
    Issue number14
    DOIs
    StatePublished - Jul 15 1991

    Keywords

    • Human anti-gp120 antibody
    • Human immunodeficiency virus 1 neutralization

    ASJC Scopus subject areas

    • General

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