Evidence for glucocorticoid regulation of the rat vasopressin V1a receptor gene

Jyoti J. Watters, Michael W. Swank, Charles W. Wilkinson, Daniel M. Dorsa

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

WRK-1 cells express vasopressin V1a receptors. Twenty-four-hour treatment of these cells with dexamethasone (DEX) resulted in an increase in [3H]AVP binding that was maximal at 12 h, and could be blocked by addition of RU 38486. The increases in [3H]AVP binding were paralleled by increases in V1a receptor mRNA. The in vivo effects of glucocorticoids (GCs) on V1a receptor binding in hepatic tissue were also investigated in adrenalectomized and hormone-replaced rats given either DEX or aldosterone (ALDO). DEX effectively increased V1a receptor binding site density whereas ALDO had no effect. The DEX effects on V1a receptor mRNA and binding strongly implicate glucocorticoids in the regulation of V1a receptor gene expression.

Original languageEnglish (US)
Pages (from-to)67-73
Number of pages7
JournalPeptides
Volume17
Issue number1
DOIs
StatePublished - 1996

Keywords

  • Adrenalectomy
  • Aldosterone
  • Glucocorticoid response element
  • Glucocorticoids
  • Liver
  • Messenger RNA
  • Receptor binding
  • Transcriptional regulation
  • WRK-1 cells

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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