Evidence for an epididymal origin of bovine sperm forward motility protein

H. Brandt, Ted Acott, D. J. Johnson, D. D. Hoskins

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Forward mobility protein (FMP) is a glycoprotein found in bovine seminal plasma that can induce forward motion in immotile caput spermatozoa under conditions of elevated cyclic AMP levels. This paper presents evidence that this seminal plasma protein is of epididymal origin. FMP activity is present in decreasing amounts in caudal epididymal fluid, seminal plasma and rete testis fluid. The FMP of caudal epididymal fluid displays properties identical to those of seminal plasma FMP after heat, freeze-thaw, trypsin or β-galactosidase treatment. Further physical evidence for the similar character of epididymal and seminal plasma FMP is shown by the fact that the complex Sepharose 6B elution profiles of the forward motility inducing activity in the 2 fluids are nearly identical. The typical motility inducing profile seen in the semen of the normal monkey was not observed in the ejaculate of the vasectomized animal. This indicates that FMP is not produced by the accessory glands. These data indicate an epididymal origin of FMP and suggest a physiological role for this protein in the acquisition of sperm motility.

Original languageEnglish (US)
Pages (from-to)830-835
Number of pages6
JournalBiology of Reproduction
Volume19
Issue number4
StatePublished - 1978

Fingerprint

Molecular Motor Proteins
Sperm Motility
Semen
Proteins
Seminal Plasma Proteins
Rete Testis
Galactosidases
Cyclic AMP
Sepharose
Trypsin
Haplorhini
Spermatozoa
Glycoproteins
Hot Temperature

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Brandt, H., Acott, T., Johnson, D. J., & Hoskins, D. D. (1978). Evidence for an epididymal origin of bovine sperm forward motility protein. Biology of Reproduction, 19(4), 830-835.

Evidence for an epididymal origin of bovine sperm forward motility protein. / Brandt, H.; Acott, Ted; Johnson, D. J.; Hoskins, D. D.

In: Biology of Reproduction, Vol. 19, No. 4, 1978, p. 830-835.

Research output: Contribution to journalArticle

Brandt, H, Acott, T, Johnson, DJ & Hoskins, DD 1978, 'Evidence for an epididymal origin of bovine sperm forward motility protein', Biology of Reproduction, vol. 19, no. 4, pp. 830-835.
Brandt, H. ; Acott, Ted ; Johnson, D. J. ; Hoskins, D. D. / Evidence for an epididymal origin of bovine sperm forward motility protein. In: Biology of Reproduction. 1978 ; Vol. 19, No. 4. pp. 830-835.
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