Evidence for an active dimer of Escherichia coli β galactosidase

C. M. Kaneshiro, C. A. Enns, M. G. Hahn, J. S. Peterson, F. J. Reithel

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7 Scopus citations

Abstract

β Galactosidase (EC 3.2.1.23), prepared from strains ML 308 and K12 3300 of E. coli, dissociated into an inactive monomer in the presence of Ag+. When such a monomer preparation is treated with excess of thiol an enzymically active dimer is formed in addition to an active tetramer. It is suggested that Ag+ may be of value in studies of other multimeric proteins as a mild dissociating agent.

Original languageEnglish (US)
Pages (from-to)433-434
Number of pages2
JournalUnknown Journal
Volume151
Issue number2
DOIs
StatePublished - Jan 1 1975

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Kaneshiro, C. M., Enns, C. A., Hahn, M. G., Peterson, J. S., & Reithel, F. J. (1975). Evidence for an active dimer of Escherichia coli β galactosidase. Unknown Journal, 151(2), 433-434. https://doi.org/10.1042/bj1510433