Evidence for an active dimer of Escherichia coli β galactosidase

C. M. Kaneshiro, Caroline Enns, M. G. Hahn, J. S. Peterson, F. J. Reithel

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

β Galactosidase (EC 3.2.1.23), prepared from strains ML 308 and K12 3300 of E. coli, dissociated into an inactive monomer in the presence of Ag +. When such a monomer preparation is treated with excess of thiol an enzymically active dimer is formed in addition to an active tetramer. It is suggested that Ag + may be of value in studies of other multimeric proteins as a mild dissociating agent.

Original languageEnglish (US)
Pages (from-to)433-434
Number of pages2
JournalBiochemical Journal
Volume151
Issue number2
StatePublished - 1975
Externally publishedYes

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Galactosidases
Escherichia coli K12
Sulfhydryl Compounds
Dimers
Escherichia coli
Monomers
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kaneshiro, C. M., Enns, C., Hahn, M. G., Peterson, J. S., & Reithel, F. J. (1975). Evidence for an active dimer of Escherichia coli β galactosidase. Biochemical Journal, 151(2), 433-434.

Evidence for an active dimer of Escherichia coli β galactosidase. / Kaneshiro, C. M.; Enns, Caroline; Hahn, M. G.; Peterson, J. S.; Reithel, F. J.

In: Biochemical Journal, Vol. 151, No. 2, 1975, p. 433-434.

Research output: Contribution to journalArticle

Kaneshiro, CM, Enns, C, Hahn, MG, Peterson, JS & Reithel, FJ 1975, 'Evidence for an active dimer of Escherichia coli β galactosidase', Biochemical Journal, vol. 151, no. 2, pp. 433-434.
Kaneshiro CM, Enns C, Hahn MG, Peterson JS, Reithel FJ. Evidence for an active dimer of Escherichia coli β galactosidase. Biochemical Journal. 1975;151(2):433-434.
Kaneshiro, C. M. ; Enns, Caroline ; Hahn, M. G. ; Peterson, J. S. ; Reithel, F. J. / Evidence for an active dimer of Escherichia coli β galactosidase. In: Biochemical Journal. 1975 ; Vol. 151, No. 2. pp. 433-434.
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