Evidence for a potential role of neuropeptide Y in ovine corpus luteum function

C. S. Keator, E. E. Custer, T. A. Hoagland, D. T. Schreiber, K. Mah, A. M. Lawson, Ov Slayden, J. A. McCracken

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Neuropeptide Y (NPY) is a neurohormone that is typically associated with food intake, but it has also been reported to affect the production of progesterone from luteal tissue in vitro. However, NPY has not been previously immunolocalized in the ovine ovary or in the corpus luteum (CL) of any species, and the effects of this neurohormone on luteal function in vivo are not known. Thus, we performed fluorescent immunohistochemistry (IHC) to localize NPY in the ovine ovary and used avidin-biotin immunocytochemistry (ICC) to further define the intracellular localization within follicles and the CL. We then infused NPY directly into the arterial supply of the autotransplanted ovaries of sheep to determine the in vivo effect of exogenous NPY on ovarian blood flow and on the luteal secretion rate of progesterone and oxytocin. Immunohistochemistry revealed that the NPY antigen was localized to cells within the follicles and CL, in the nerve fibers of the ovarian stroma, and in the vessels of the ovarian hilus. In the follicle, the NPY antigen was localized to nerves and vessels within the theca interna layer, and strong staining was observed in the granulosal cells of antral follicles. In the CL, NPY was localized in large luteal cells and in the vascular pericytes and/or endothelial cells of blood vessels, found dispersed throughout the gland and within the luteal capsule. In vivo incremental infusions of NPY at 1, 10, 100, and 1,000 ng/min, each for a 30-min period, into the arterial supply of the transplanted ovary of sheep bearing a CL 11 d of age increased (P ≤ 0.05) ovarian blood flow. The intra-arterial infusions of NPY also increased (P ≤ 0.05) in a dose-dependent manner the secretion rate of oxytocin, which was positively correlated (P ≤ 0.05) with the observed increase in ovarian blood flow. The infusions of NPY had a minimal effect on the secretion rate of progesterone, and similar intra-arterial infusions of NPY into sheep with ovarian transplants bearing a CL over 30 d of age had no significant effect on ovarian blood flow or on the secretion rate of progesterone. These results suggest that NPY acts on the luteal vascular system and the large luteal cells to rapidly stimulate blood flow and the secretion of oxytocin, respectively, which collectively implies a putative role for NPY during the process of luteolysis when increasing amounts of oxytocin are secreted from the ovine CL in response to uterine pulses of prostaglandin F2α.

    Original languageEnglish (US)
    Pages (from-to)103-114
    Number of pages12
    JournalDomestic Animal Endocrinology
    Volume38
    Issue number2
    DOIs
    StatePublished - Feb 2010

    Fingerprint

    neuropeptide Y
    Neuropeptide Y
    Corpus Luteum
    corpus luteum
    Sheep
    sheep
    blood flow
    oxytocin
    Oxytocin
    secretion
    Progesterone
    Ovary
    progesterone
    neurohormones
    Blood Vessels
    Luteal Cells
    luteal cells
    Intra Arterial Infusions
    Immunohistochemistry
    blood vessels

    Keywords

    • Corpus luteum
    • Neuropeptide Y
    • Ovary
    • Oxytocin
    • Progesterone
    • Sheep

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • Endocrinology
    • Food Animals

    Cite this

    Keator, C. S., Custer, E. E., Hoagland, T. A., Schreiber, D. T., Mah, K., Lawson, A. M., ... McCracken, J. A. (2010). Evidence for a potential role of neuropeptide Y in ovine corpus luteum function. Domestic Animal Endocrinology, 38(2), 103-114. https://doi.org/10.1016/j.domaniend.2009.08.003

    Evidence for a potential role of neuropeptide Y in ovine corpus luteum function. / Keator, C. S.; Custer, E. E.; Hoagland, T. A.; Schreiber, D. T.; Mah, K.; Lawson, A. M.; Slayden, Ov; McCracken, J. A.

    In: Domestic Animal Endocrinology, Vol. 38, No. 2, 02.2010, p. 103-114.

    Research output: Contribution to journalArticle

    Keator, CS, Custer, EE, Hoagland, TA, Schreiber, DT, Mah, K, Lawson, AM, Slayden, O & McCracken, JA 2010, 'Evidence for a potential role of neuropeptide Y in ovine corpus luteum function', Domestic Animal Endocrinology, vol. 38, no. 2, pp. 103-114. https://doi.org/10.1016/j.domaniend.2009.08.003
    Keator, C. S. ; Custer, E. E. ; Hoagland, T. A. ; Schreiber, D. T. ; Mah, K. ; Lawson, A. M. ; Slayden, Ov ; McCracken, J. A. / Evidence for a potential role of neuropeptide Y in ovine corpus luteum function. In: Domestic Animal Endocrinology. 2010 ; Vol. 38, No. 2. pp. 103-114.
    @article{4e9f180e53304efa9e4ecf6d489a7c31,
    title = "Evidence for a potential role of neuropeptide Y in ovine corpus luteum function",
    abstract = "Neuropeptide Y (NPY) is a neurohormone that is typically associated with food intake, but it has also been reported to affect the production of progesterone from luteal tissue in vitro. However, NPY has not been previously immunolocalized in the ovine ovary or in the corpus luteum (CL) of any species, and the effects of this neurohormone on luteal function in vivo are not known. Thus, we performed fluorescent immunohistochemistry (IHC) to localize NPY in the ovine ovary and used avidin-biotin immunocytochemistry (ICC) to further define the intracellular localization within follicles and the CL. We then infused NPY directly into the arterial supply of the autotransplanted ovaries of sheep to determine the in vivo effect of exogenous NPY on ovarian blood flow and on the luteal secretion rate of progesterone and oxytocin. Immunohistochemistry revealed that the NPY antigen was localized to cells within the follicles and CL, in the nerve fibers of the ovarian stroma, and in the vessels of the ovarian hilus. In the follicle, the NPY antigen was localized to nerves and vessels within the theca interna layer, and strong staining was observed in the granulosal cells of antral follicles. In the CL, NPY was localized in large luteal cells and in the vascular pericytes and/or endothelial cells of blood vessels, found dispersed throughout the gland and within the luteal capsule. In vivo incremental infusions of NPY at 1, 10, 100, and 1,000 ng/min, each for a 30-min period, into the arterial supply of the transplanted ovary of sheep bearing a CL 11 d of age increased (P ≤ 0.05) ovarian blood flow. The intra-arterial infusions of NPY also increased (P ≤ 0.05) in a dose-dependent manner the secretion rate of oxytocin, which was positively correlated (P ≤ 0.05) with the observed increase in ovarian blood flow. The infusions of NPY had a minimal effect on the secretion rate of progesterone, and similar intra-arterial infusions of NPY into sheep with ovarian transplants bearing a CL over 30 d of age had no significant effect on ovarian blood flow or on the secretion rate of progesterone. These results suggest that NPY acts on the luteal vascular system and the large luteal cells to rapidly stimulate blood flow and the secretion of oxytocin, respectively, which collectively implies a putative role for NPY during the process of luteolysis when increasing amounts of oxytocin are secreted from the ovine CL in response to uterine pulses of prostaglandin F2α.",
    keywords = "Corpus luteum, Neuropeptide Y, Ovary, Oxytocin, Progesterone, Sheep",
    author = "Keator, {C. S.} and Custer, {E. E.} and Hoagland, {T. A.} and Schreiber, {D. T.} and K. Mah and Lawson, {A. M.} and Ov Slayden and McCracken, {J. A.}",
    year = "2010",
    month = "2",
    doi = "10.1016/j.domaniend.2009.08.003",
    language = "English (US)",
    volume = "38",
    pages = "103--114",
    journal = "Domestic Animal Endocrinology",
    issn = "0739-7240",
    publisher = "Elsevier Inc.",
    number = "2",

    }

    TY - JOUR

    T1 - Evidence for a potential role of neuropeptide Y in ovine corpus luteum function

    AU - Keator, C. S.

    AU - Custer, E. E.

    AU - Hoagland, T. A.

    AU - Schreiber, D. T.

    AU - Mah, K.

    AU - Lawson, A. M.

    AU - Slayden, Ov

    AU - McCracken, J. A.

    PY - 2010/2

    Y1 - 2010/2

    N2 - Neuropeptide Y (NPY) is a neurohormone that is typically associated with food intake, but it has also been reported to affect the production of progesterone from luteal tissue in vitro. However, NPY has not been previously immunolocalized in the ovine ovary or in the corpus luteum (CL) of any species, and the effects of this neurohormone on luteal function in vivo are not known. Thus, we performed fluorescent immunohistochemistry (IHC) to localize NPY in the ovine ovary and used avidin-biotin immunocytochemistry (ICC) to further define the intracellular localization within follicles and the CL. We then infused NPY directly into the arterial supply of the autotransplanted ovaries of sheep to determine the in vivo effect of exogenous NPY on ovarian blood flow and on the luteal secretion rate of progesterone and oxytocin. Immunohistochemistry revealed that the NPY antigen was localized to cells within the follicles and CL, in the nerve fibers of the ovarian stroma, and in the vessels of the ovarian hilus. In the follicle, the NPY antigen was localized to nerves and vessels within the theca interna layer, and strong staining was observed in the granulosal cells of antral follicles. In the CL, NPY was localized in large luteal cells and in the vascular pericytes and/or endothelial cells of blood vessels, found dispersed throughout the gland and within the luteal capsule. In vivo incremental infusions of NPY at 1, 10, 100, and 1,000 ng/min, each for a 30-min period, into the arterial supply of the transplanted ovary of sheep bearing a CL 11 d of age increased (P ≤ 0.05) ovarian blood flow. The intra-arterial infusions of NPY also increased (P ≤ 0.05) in a dose-dependent manner the secretion rate of oxytocin, which was positively correlated (P ≤ 0.05) with the observed increase in ovarian blood flow. The infusions of NPY had a minimal effect on the secretion rate of progesterone, and similar intra-arterial infusions of NPY into sheep with ovarian transplants bearing a CL over 30 d of age had no significant effect on ovarian blood flow or on the secretion rate of progesterone. These results suggest that NPY acts on the luteal vascular system and the large luteal cells to rapidly stimulate blood flow and the secretion of oxytocin, respectively, which collectively implies a putative role for NPY during the process of luteolysis when increasing amounts of oxytocin are secreted from the ovine CL in response to uterine pulses of prostaglandin F2α.

    AB - Neuropeptide Y (NPY) is a neurohormone that is typically associated with food intake, but it has also been reported to affect the production of progesterone from luteal tissue in vitro. However, NPY has not been previously immunolocalized in the ovine ovary or in the corpus luteum (CL) of any species, and the effects of this neurohormone on luteal function in vivo are not known. Thus, we performed fluorescent immunohistochemistry (IHC) to localize NPY in the ovine ovary and used avidin-biotin immunocytochemistry (ICC) to further define the intracellular localization within follicles and the CL. We then infused NPY directly into the arterial supply of the autotransplanted ovaries of sheep to determine the in vivo effect of exogenous NPY on ovarian blood flow and on the luteal secretion rate of progesterone and oxytocin. Immunohistochemistry revealed that the NPY antigen was localized to cells within the follicles and CL, in the nerve fibers of the ovarian stroma, and in the vessels of the ovarian hilus. In the follicle, the NPY antigen was localized to nerves and vessels within the theca interna layer, and strong staining was observed in the granulosal cells of antral follicles. In the CL, NPY was localized in large luteal cells and in the vascular pericytes and/or endothelial cells of blood vessels, found dispersed throughout the gland and within the luteal capsule. In vivo incremental infusions of NPY at 1, 10, 100, and 1,000 ng/min, each for a 30-min period, into the arterial supply of the transplanted ovary of sheep bearing a CL 11 d of age increased (P ≤ 0.05) ovarian blood flow. The intra-arterial infusions of NPY also increased (P ≤ 0.05) in a dose-dependent manner the secretion rate of oxytocin, which was positively correlated (P ≤ 0.05) with the observed increase in ovarian blood flow. The infusions of NPY had a minimal effect on the secretion rate of progesterone, and similar intra-arterial infusions of NPY into sheep with ovarian transplants bearing a CL over 30 d of age had no significant effect on ovarian blood flow or on the secretion rate of progesterone. These results suggest that NPY acts on the luteal vascular system and the large luteal cells to rapidly stimulate blood flow and the secretion of oxytocin, respectively, which collectively implies a putative role for NPY during the process of luteolysis when increasing amounts of oxytocin are secreted from the ovine CL in response to uterine pulses of prostaglandin F2α.

    KW - Corpus luteum

    KW - Neuropeptide Y

    KW - Ovary

    KW - Oxytocin

    KW - Progesterone

    KW - Sheep

    UR - http://www.scopus.com/inward/record.url?scp=72549094675&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=72549094675&partnerID=8YFLogxK

    U2 - 10.1016/j.domaniend.2009.08.003

    DO - 10.1016/j.domaniend.2009.08.003

    M3 - Article

    VL - 38

    SP - 103

    EP - 114

    JO - Domestic Animal Endocrinology

    JF - Domestic Animal Endocrinology

    SN - 0739-7240

    IS - 2

    ER -