TY - JOUR
T1 - Evaluation of three different decontamination techniques on biofilm formation, and on physical and chemical properties of resin composites
AU - André, Carolina Bosso
AU - dos Santos, Andressa
AU - Pfeifer, Carmem Silvia
AU - Giannini, Marcelo
AU - Girotto, Emerson Marcelo
AU - Ferracane, Jack Liborio
N1 - Publisher Copyright:
© 2017 Wiley Periodicals, Inc.
PY - 2018/4
Y1 - 2018/4
N2 - Objectives: This study evaluated three different sterilization/disinfection techniques for resin composites on bacterial growth and surface modification after decontamination. Methods: Two resin composites were sterilized/disinfected with three different techniques: UV light, 1% chloramine T, and 70% ethanol. Four different times were used for each technique to determine the shortest time that the solution or UV light was effective. The influence of sterilization/disinfection technique on bacterial growth was evaluated by analyzing the metabolic activity, using the AlamarBlue™ assay, bacterial viability, and SEM images from biofilms of Streptococcus mutans. The surface change, after the process, was analyzed with ATR/FTIR and SEM images. The solutions used for decontamination (1% chloramine-T and 70% ethanol) were analyzed with 1 H-NMR to identify any resin compounds leached during the process. Results: One minute of decontamination was efficient for all three methods tested. Chloramine-T increased the surface porosity on resin composites, no changes were observed for UV light and 70% ethanol, however, 1 H-NMR identified leached monomers only when 70% ethanol was used. No chemical change of the materials was found under ATR/FTIR analyses after the decontamination process. Chloramine-T, with no previous wash, increased the bacterial viability for both resin composites and increased the bacterial metabolism for the resin composite without fluoride. Conclusion: UV light had no interference on the resin composites properties tested using 1 min of exposure compared to the other decontamination methods.
AB - Objectives: This study evaluated three different sterilization/disinfection techniques for resin composites on bacterial growth and surface modification after decontamination. Methods: Two resin composites were sterilized/disinfected with three different techniques: UV light, 1% chloramine T, and 70% ethanol. Four different times were used for each technique to determine the shortest time that the solution or UV light was effective. The influence of sterilization/disinfection technique on bacterial growth was evaluated by analyzing the metabolic activity, using the AlamarBlue™ assay, bacterial viability, and SEM images from biofilms of Streptococcus mutans. The surface change, after the process, was analyzed with ATR/FTIR and SEM images. The solutions used for decontamination (1% chloramine-T and 70% ethanol) were analyzed with 1 H-NMR to identify any resin compounds leached during the process. Results: One minute of decontamination was efficient for all three methods tested. Chloramine-T increased the surface porosity on resin composites, no changes were observed for UV light and 70% ethanol, however, 1 H-NMR identified leached monomers only when 70% ethanol was used. No chemical change of the materials was found under ATR/FTIR analyses after the decontamination process. Chloramine-T, with no previous wash, increased the bacterial viability for both resin composites and increased the bacterial metabolism for the resin composite without fluoride. Conclusion: UV light had no interference on the resin composites properties tested using 1 min of exposure compared to the other decontamination methods.
KW - biofilm
KW - resin composite
KW - sterilization
KW - surface analysis
UR - http://www.scopus.com/inward/record.url?scp=85018305201&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85018305201&partnerID=8YFLogxK
U2 - 10.1002/jbm.b.33907
DO - 10.1002/jbm.b.33907
M3 - Article
C2 - 28440891
AN - SCOPUS:85018305201
SN - 1552-4973
VL - 106
SP - 945
EP - 953
JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials
JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials
IS - 3
ER -