Objectives: This study evaluated three different sterilization/disinfection techniques for resin composites on bacterial growth and surface modification after decontamination. Methods: Two resin composites were sterilized/disinfected with three different techniques: UV light, 1% chloramine T, and 70% ethanol. Four different times were used for each technique to determine the shortest time that the solution or UV light was effective. The influence of sterilization/disinfection technique on bacterial growth was evaluated by analyzing the metabolic activity, using the AlamarBlue™ assay, bacterial viability, and SEM images from biofilms of Streptococcus mutans. The surface change, after the process, was analyzed with ATR/FTIR and SEM images. The solutions used for decontamination (1% chloramine-T and 70% ethanol) were analyzed with 1H-NMR to identify any resin compounds leached during the process. Results: One minute of decontamination was efficient for all three methods tested. Chloramine-T increased the surface porosity on resin composites, no changes were observed for UV light and 70% ethanol, however, 1H-NMR identified leached monomers only when 70% ethanol was used. No chemical change of the materials was found under ATR/FTIR analyses after the decontamination process. Chloramine-T, with no previous wash, increased the bacterial viability for both resin composites and increased the bacterial metabolism for the resin composite without fluoride. Conclusion: UV light had no interference on the resin composites properties tested using 1 min of exposure compared to the other decontamination methods.
|Original language||English (US)|
|Journal||Journal of Biomedical Materials Research - Part B Applied Biomaterials|
|Publication status||Accepted/In press - 2017|
- Resin composite
- Surface analysis
ASJC Scopus subject areas
- Biomedical Engineering