Evaluation of the effects of 17β-estradiol (17β-E2) on gene expression in experimental autoimmune encephalomyelitis using DNA microarray

Agata Matejuk, Jami Dwyer, Alex Zamora, Arthur Vandenbark, Halina Offner

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Abstract

The aim of this study was to identify immune-related genes affected by treatment with 17β-estradiol (17β-E2) that contribute to protection of T cell antigen receptor double transgenic mice from experimental autoimmune encephalomyelitis (EAE). The Affymetrix microarray system was used to screen more than 12,000 genes from E2-treated mice protected from EAE vs. control mice with severe EAE. In general, E2 treatment affected about 10% of the genes tested, but only 18 cytokine, chemokine/receptor, adhesion molecule, or activation genes were up- or down-regulated more than 2.4-fold by E2 treatment. Down-regulated genes included TNFα (an important proinflammatory cytokine in EAE); peptidoglycan recognition proteins (Pgrp); regulated on activation, normal T cell expressed and secreted (RANTES); and neural cell adhesion molecule (MCP-1). Up-regulated genes included cytotoxic T lymphocyte antigen-4 (CTLA-4; known to inhibit T cell activation), TGF β3, IL-18, and two interferon-γ-induced genes, the chemokines: monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1β (MIP-1β), vascular cell adhesion molecule (VCAM), and disintegrin metalloprotease (thought to regulate TNFα production). These results implicate a limited set of known and previously unsuspected E2-sensitive genes that may be crucial for inhibition of EAE and potentially the human disease, multiple sclerosis.

Original languageEnglish (US)
Pages (from-to)313-319
Number of pages7
JournalEndocrinology
Volume143
Issue number1
DOIs
StatePublished - Jan 1 2002

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Autoimmune Experimental Encephalomyelitis
Oligonucleotide Array Sequence Analysis
Estradiol
Gene Expression
Genes
Chemokine CCL2
CTLA-4 Antigen
T-Lymphocytes
Disintegrins
Macrophage Inflammatory Proteins
Neural Cell Adhesion Molecules
Interleukin-18
Cytokine Receptors
Vascular Cell Adhesion Molecule-1
Chemokine Receptors
Metalloproteases
T-Cell Antigen Receptor
Chemokines
Interferons
Transgenic Mice

ASJC Scopus subject areas

  • Endocrinology

Cite this

Evaluation of the effects of 17β-estradiol (17β-E2) on gene expression in experimental autoimmune encephalomyelitis using DNA microarray. / Matejuk, Agata; Dwyer, Jami; Zamora, Alex; Vandenbark, Arthur; Offner, Halina.

In: Endocrinology, Vol. 143, No. 1, 01.01.2002, p. 313-319.

Research output: Contribution to journalArticle

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