Evaluation of surface structural and mechanical changes following remineralization of dentin

Luiz Bertassoni, Stefan Habelitz, Megan Pugach, Paulo Cesar Soares, Sally Jean Marshall, Grayson William Marshall

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

This study sought to gain insights into the surface structural and mechanical changes leading to remineralization of dentin. Remineralization was compared between a continuous remineralization approach and a nonbuffered static approach using solutions of the same initial composition. Artificial carious lesions were treated for 5 days and analyzed every 24 h using nanoindentation in water, SEM, and AFM. The continuous approach yielded a recovery of mechanical properties of up to 60% of normal dentin, whereas the static approach led to recovery of only 10%. Image analysis revealed that the static approach yielded the formation of areas suggestive of an apatite precipitate on the surface of the dentin matrix. In contrast, surface precipitate was absent using the continuous approach, suggesting that mineral formed within the lesion and re-associated with the collagenous matrix. This study provided evidence that mechanical recovery of dentin in near physiological conditions is attainable through the continuous delivery of calcium and phosphate ions.

Original languageEnglish (US)
Pages (from-to)312-319
Number of pages8
JournalScanning
Volume32
Issue number5
DOIs
StatePublished - Sep 2010
Externally publishedYes

Fingerprint

recovery
Recovery
lesions
evaluation
Precipitates
precipitates
Apatite
apatites
Nanoindentation
matrices
nanoindentation
image analysis
Image analysis
calcium
Calcium
delivery
phosphates
Phosphates
Minerals
minerals

Keywords

  • AFM
  • collagen
  • dentin
  • hydroxyapatite
  • mechanical properties
  • nanoindentation
  • remineralization

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Bertassoni, L., Habelitz, S., Pugach, M., Soares, P. C., Marshall, S. J., & Marshall, G. W. (2010). Evaluation of surface structural and mechanical changes following remineralization of dentin. Scanning, 32(5), 312-319. https://doi.org/10.1002/sca.20199

Evaluation of surface structural and mechanical changes following remineralization of dentin. / Bertassoni, Luiz; Habelitz, Stefan; Pugach, Megan; Soares, Paulo Cesar; Marshall, Sally Jean; Marshall, Grayson William.

In: Scanning, Vol. 32, No. 5, 09.2010, p. 312-319.

Research output: Contribution to journalArticle

Bertassoni, L, Habelitz, S, Pugach, M, Soares, PC, Marshall, SJ & Marshall, GW 2010, 'Evaluation of surface structural and mechanical changes following remineralization of dentin', Scanning, vol. 32, no. 5, pp. 312-319. https://doi.org/10.1002/sca.20199
Bertassoni, Luiz ; Habelitz, Stefan ; Pugach, Megan ; Soares, Paulo Cesar ; Marshall, Sally Jean ; Marshall, Grayson William. / Evaluation of surface structural and mechanical changes following remineralization of dentin. In: Scanning. 2010 ; Vol. 32, No. 5. pp. 312-319.
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