Evaluation of spherical particle sizes with an asymmetric illumination microscope

Jessica C. Ramella-Roman; Paulo R. Bargo; Scott A. Prahl; Steven L. Jacques

doi:10.1109/JSTQE.2003.811289

Evaluation of spherical particle sizes with an asymmetric illumination microscope

Jessica C. Ramella-Roman, Paulo R. Bargo, Scott A. Prahl, Steven L. Jacques

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A polarized microscope system is used to perform goniometric measurements of light scattered by small particles. The light incident angle on a sample of monodispersed latex microspheres is increased sequentially and a microscope objective lens collects scattered light from the samples. Light is only collected at angles greater than the objective lens numerical aperture (NA) so that only light scattered by the spheres is collected. The experimental results were modeled with a Mie theory-based algorithm. Experiments conducted with microspheres of diameter 1.03, 2.03, and 6.4 μm show that, by decreasing the objective lens NA from NA = 0.55 to NA = 0.0548, a more distinguishable scattering pattern is detectable. From these highly shaped curves, we found that the size of a sphere of nominal diameter 2.03 μm was 2.11 ±0.06 μm and a 6.4 μm sphere was 6.34 ±0.07 μm.

Original language	English (US)
Pages (from-to)	301-306
Number of pages	6
Journal	IEEE Journal on Selected Topics in Quantum Electronics
Volume	9
Issue number	2
DOIs	https://doi.org/10.1109/JSTQE.2003.811289
State	Published - Mar 2003

Keywords

Asymmetric illumination
Goniometry
Mie theory
Oblique microscopy
Polarized light microscopy

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering

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10.1109/JSTQE.2003.811289

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title = "Evaluation of spherical particle sizes with an asymmetric illumination microscope",

abstract = "A polarized microscope system is used to perform goniometric measurements of light scattered by small particles. The light incident angle on a sample of monodispersed latex microspheres is increased sequentially and a microscope objective lens collects scattered light from the samples. Light is only collected at angles greater than the objective lens numerical aperture (NA) so that only light scattered by the spheres is collected. The experimental results were modeled with a Mie theory-based algorithm. Experiments conducted with microspheres of diameter 1.03, 2.03, and 6.4 μm show that, by decreasing the objective lens NA from NA = 0.55 to NA = 0.0548, a more distinguishable scattering pattern is detectable. From these highly shaped curves, we found that the size of a sphere of nominal diameter 2.03 μm was 2.11 ±0.06 μm and a 6.4 μm sphere was 6.34 ±0.07 μm.",

keywords = "Asymmetric illumination, Goniometry, Mie theory, Oblique microscopy, Polarized light microscopy",

author = "Ramella-Roman, {Jessica C.} and Bargo, {Paulo R.} and Prahl, {Scott A.} and Jacques, {Steven L.}",

note = "Funding Information: Manuscript received November 18, 2002; revised February 3, 2003. This work was sponsored by the National Institute of Health under Grant RO1-CA80985 and Grant R24-CA84587. The authors are with the Oregon Health and Sciences University, Portland, OR 97225 USA (e-mail: sjacques@ece.ogi.edu). Digital Object Identifier 10.1109/JSTQE.2003.811289",

year = "2003",

month = mar,

doi = "10.1109/JSTQE.2003.811289",

language = "English (US)",

volume = "9",

pages = "301--306",

journal = "IEEE Journal on Selected Topics in Quantum Electronics",

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AU - Ramella-Roman, Jessica C.

AU - Bargo, Paulo R.

AU - Prahl, Scott A.

AU - Jacques, Steven L.

N1 - Funding Information: Manuscript received November 18, 2002; revised February 3, 2003. This work was sponsored by the National Institute of Health under Grant RO1-CA80985 and Grant R24-CA84587. The authors are with the Oregon Health and Sciences University, Portland, OR 97225 USA (e-mail: sjacques@ece.ogi.edu). Digital Object Identifier 10.1109/JSTQE.2003.811289

PY - 2003/3

Y1 - 2003/3

N2 - A polarized microscope system is used to perform goniometric measurements of light scattered by small particles. The light incident angle on a sample of monodispersed latex microspheres is increased sequentially and a microscope objective lens collects scattered light from the samples. Light is only collected at angles greater than the objective lens numerical aperture (NA) so that only light scattered by the spheres is collected. The experimental results were modeled with a Mie theory-based algorithm. Experiments conducted with microspheres of diameter 1.03, 2.03, and 6.4 μm show that, by decreasing the objective lens NA from NA = 0.55 to NA = 0.0548, a more distinguishable scattering pattern is detectable. From these highly shaped curves, we found that the size of a sphere of nominal diameter 2.03 μm was 2.11 ±0.06 μm and a 6.4 μm sphere was 6.34 ±0.07 μm.

AB - A polarized microscope system is used to perform goniometric measurements of light scattered by small particles. The light incident angle on a sample of monodispersed latex microspheres is increased sequentially and a microscope objective lens collects scattered light from the samples. Light is only collected at angles greater than the objective lens numerical aperture (NA) so that only light scattered by the spheres is collected. The experimental results were modeled with a Mie theory-based algorithm. Experiments conducted with microspheres of diameter 1.03, 2.03, and 6.4 μm show that, by decreasing the objective lens NA from NA = 0.55 to NA = 0.0548, a more distinguishable scattering pattern is detectable. From these highly shaped curves, we found that the size of a sphere of nominal diameter 2.03 μm was 2.11 ±0.06 μm and a 6.4 μm sphere was 6.34 ±0.07 μm.

KW - Asymmetric illumination

KW - Goniometry

KW - Mie theory

KW - Oblique microscopy

KW - Polarized light microscopy

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Evaluation of spherical particle sizes with an asymmetric illumination microscope

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