Evaluation of four gentamicin and tobramycin assay procedures for clinical laboratories

F. G. Witebsky, C. A. Sliva, S. T. Selepak, M. E. Ruddel, J. D. MacLowry, E. E. Johnson, R. J. Elin

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Abstract

Accuracy, precision, and clinical laboratory utility of the TDX (Abbott Laboratories), Auto-ICS (Beckman Instruments, Inc.), COBAS-Bio (Roche Analytical Instruments Inc.) with reagent kits (Syva), and EMIT (Syva) for gentamicin and tobramycin serum assay were assessed. TDX, COBAS-Bio, and EMIT analytical systems showed a proportional bias of less than 10% for recovery studies and a coefficient of variation less than 5% for within-run precision. The results of the recovery studies with the Auto-ICS showed a proportional bias of 25% with gentamicin and 16% with tobramycin. The within-run precision expressed as the coefficient of variation for the Auto-ICS was 6.7% for gentamicin and 8.6% for tobramycin. In comparisons involving gentamicin- and tobramycin-containing patient samples, the results with the TDX analytical system showed the best agreement with the COBAS-Bio. For the determination of these two antibiotics, the TDX analytical system provided the best overall accuracy and precision.

Original languageEnglish (US)
Pages (from-to)890-894
Number of pages5
JournalJournal of Clinical Microbiology
Volume18
Issue number4
StatePublished - Nov 26 1983

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ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Witebsky, F. G., Sliva, C. A., Selepak, S. T., Ruddel, M. E., MacLowry, J. D., Johnson, E. E., & Elin, R. J. (1983). Evaluation of four gentamicin and tobramycin assay procedures for clinical laboratories. Journal of Clinical Microbiology, 18(4), 890-894.