TY - JOUR
T1 - Evaluation of five gentamicin assay procedures for clinical microbiology laboratories
AU - Selepak, S. T.
AU - Witebsky, F. G.
AU - Robertson, E. A.
AU - MacLowry, J. D.
PY - 1981
Y1 - 1981
N2 - Five gentamicin assay procedures (a bioassay, an enzyme immunoassay, a latex agglutination inhibition test, a fluorescence immunoassay, and a radioimmunoassay) were evaluated to determine which was optimal for our laboratory. The evaluation was based on recovery and precision studies and results of analyses of patient samples, as well as technical assay performance factors. The latex agglutination inhibition test appears useful for laboratories performing only occasional assays for gentamicin; however, the fact that some rheumatoid factor-positive sera, as well as some other sera for unknown reasons, may give falsely low values is a potential drawback to this procedure. Because of its accuracy, precision, rapid turn-around time, and relative simplicity of performance, we selected the enzyme immunoassay procedure for routine use for gentamicin assays in our laboratory.
AB - Five gentamicin assay procedures (a bioassay, an enzyme immunoassay, a latex agglutination inhibition test, a fluorescence immunoassay, and a radioimmunoassay) were evaluated to determine which was optimal for our laboratory. The evaluation was based on recovery and precision studies and results of analyses of patient samples, as well as technical assay performance factors. The latex agglutination inhibition test appears useful for laboratories performing only occasional assays for gentamicin; however, the fact that some rheumatoid factor-positive sera, as well as some other sera for unknown reasons, may give falsely low values is a potential drawback to this procedure. Because of its accuracy, precision, rapid turn-around time, and relative simplicity of performance, we selected the enzyme immunoassay procedure for routine use for gentamicin assays in our laboratory.
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U2 - 10.1128/jcm.13.4.742-749.1981
DO - 10.1128/jcm.13.4.742-749.1981
M3 - Article
C2 - 7014624
AN - SCOPUS:0019463880
SN - 0095-1137
VL - 13
SP - 742
EP - 749
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 4
ER -