Evaluating revascularization and flap survival using vascular endothelial growth factor in an irradiated rat model

Patrick C. Angelos, Shelley R. Winn, Darryl S. Kaurin, John Holland, Mark Wax

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To evaluate the role of vascular endothelial growth factor (VEGF) plasmid DNA (pDNA) in improving flap revascularization in a previously developed rat model. Our hypothesis was that the uptake and expression of VEGF pDNA in the wound bed would improve revascularization and flap viability. Design: Twenty-eight male Sprague-Dawley rats received a total dose of 40 Gy electron beam radiation to the ventral abdominal wall. After a recovery period, they underwent a ventral fasciocutaneous flap procedure with a 2-hour ischemia period. Group 1 (n=14) received topical VEGF pDNA, in vivo cationic polymer, and fibrin sealant. Group 2 (n=14) received topical cationic polymer and fibrin sealant only. Seven of the rats from each group underwent pedicle ligation at 8 or 14 days. The primary outcome measure was percentage of flap revascularization 5 days after pedicle ligation. Results: Rats receiving VEGF pDNA had a significantly higher rate of flap revascularization (90.8% vs 79.8%) after pedicle ligation at 14 days (P=.045). At 8 days, rats receiving VEGF pDNA (group 1) had an increased rate of flap revascularization (58.2% vs 42.8%) that approached significance (P=.11). Conclusion: This study demonstrates the potential of VEGF pDNA to improve revascularization and flap viability in previously irradiated tissue.

Original languageEnglish (US)
Pages (from-to)185-189
Number of pages5
JournalArchives of Facial Plastic Surgery
Volume13
Issue number3
DOIs
StatePublished - May 2011

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Vascular Endothelial Growth Factor A
Plasmids
DNA
Ligation
Fibrin Tissue Adhesive
Polymers
Abdominal Wall
Sprague Dawley Rats
Ischemia
Outcome Assessment (Health Care)
Electrons
Radiation
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery
  • Medicine(all)

Cite this

Evaluating revascularization and flap survival using vascular endothelial growth factor in an irradiated rat model. / Angelos, Patrick C.; Winn, Shelley R.; Kaurin, Darryl S.; Holland, John; Wax, Mark.

In: Archives of Facial Plastic Surgery, Vol. 13, No. 3, 05.2011, p. 185-189.

Research output: Contribution to journalArticle

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