Evaluating optical properties of isolated biological scatterers from confocal and low-coherence images

David Levitz, Ravikant Samathan, Monica T. Hinds, Steven L. Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In biomedical optics applications, the scattering of light by biological tissue is typically mimicked by embedding microparticles such as polystyrene microspheres or TiO2 within a non-scattering matrix. Such particles are well structured and give rise to uniform optical scattering properties. However, typical biological scatterers are seldom well-organized nor uniformly sized. In this work, we sought to characterize the scattering properties from particles common to many tissues such as collagen fibers, cells, and lipids. These purified particles were suspended and sandwiched between 2 glass cover slips to form disposable phantoms. The phantoms were imaged by optical coherence tomography and reflectance-mode confocal microscopy. From the images, the attenuation and reflectivity of the sample were evaluated by fitting the depth-dependent signal from specified regions of the image to a theoretical model. The fitted attenuation and reflectivity were used to deduce a distribution of local values of the scattering coefficient and anisotropy factor for each phantom. The measured optical properties at the 2 wavelengths differed in ways that can be explained by Mie theory, suggesting that despite their complex structure, typical biological scatterers exhibit some regularity that can potentially be characterized quantitatively.

Original languageEnglish (US)
Title of host publicationDesign and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue
DOIs
StatePublished - Apr 21 2008
EventDesign and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue - San Jose, CA, United States
Duration: Jan 19 2008Jan 21 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6870
ISSN (Print)1605-7422

Other

OtherDesign and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue
CountryUnited States
CitySan Jose, CA
Period1/19/081/21/08

Keywords

  • Anisotropy
  • Cells
  • Collagen
  • Confocal microscopy
  • Lipids
  • Optical coherence tomography
  • Optical properties
  • Scattering

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Levitz, D., Samathan, R., Hinds, M. T., & Jacques, S. L. (2008). Evaluating optical properties of isolated biological scatterers from confocal and low-coherence images. In Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue [68700G] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6870). https://doi.org/10.1117/12.763776