Evaluating DSAEK graft deturgescence in preservation medium after microkeratome cut with optical coherence tomography

Maolong Tang, Christopher Stoeger, Joshua Galloway, Jeffrey Holiman, Matthew R. Bald, David Huang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: To evaluate the Descemet stripping automated endothelial keratoplasty (DSAEK) graft deturgescence in preservation medium after microkeratome cut using Fourier domain optical coherence tomography. Methods: The central and peripheral thickness of DSAEK grafts was measured by Fourier domain optical coherence tomography immediately after microkeratome cuts and 1, 2, 3, and 4 hours afterward. All measurements were taken when the grafts were stored in 4°C preservation medium. The hourly change in central graft thickness and graft shape (peripheral graft thicknes 2 central graft thickness) was calculated and tracked over time. Results: Five DSAEK grafts were measured. The average central graft thickness was 188.7 ± 44.4 μm (range, 146-255 μm) immediately after microkeratome cuts. The average central graft thickness was 147.5 ± 33.0 μm (range, 116-190 μm) after 4 hours in preservation medium (P <0.001). The average hourly change in central graft thickness was 230.5 mm (P = 0.0051), 28.6 mm (P = 0.055), 22.0 μm (P = 0.42), and 0.0 μm (P = 0.93) at 1, 2, 3, and 4 hours, respectively, after microkeratome cuts. The average hourly change in graft shape was insignificant. Conclusions: DSAEK grafts become thinner after microkeratome cut and stabilize at approximately 2 hours. Therefore, DSAEK graft thickness should be measured at 1.5 to 3 hours after microkeratome cut.

Original languageEnglish (US)
Pages (from-to)847-850
Number of pages4
JournalCornea
Volume32
Issue number6
DOIs
StatePublished - Jun 2013

Fingerprint

Descemet Stripping Endothelial Keratoplasty
Optical Coherence Tomography
Transplants

Keywords

  • Corneal deturgescence
  • Eye bank
  • Optical coherence tomography

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Evaluating DSAEK graft deturgescence in preservation medium after microkeratome cut with optical coherence tomography. / Tang, Maolong; Stoeger, Christopher; Galloway, Joshua; Holiman, Jeffrey; Bald, Matthew R.; Huang, David.

In: Cornea, Vol. 32, No. 6, 06.2013, p. 847-850.

Research output: Contribution to journalArticle

Tang, Maolong ; Stoeger, Christopher ; Galloway, Joshua ; Holiman, Jeffrey ; Bald, Matthew R. ; Huang, David. / Evaluating DSAEK graft deturgescence in preservation medium after microkeratome cut with optical coherence tomography. In: Cornea. 2013 ; Vol. 32, No. 6. pp. 847-850.
@article{39062d1c81254c9ca43daa958afbbdbd,
title = "Evaluating DSAEK graft deturgescence in preservation medium after microkeratome cut with optical coherence tomography",
abstract = "Purpose: To evaluate the Descemet stripping automated endothelial keratoplasty (DSAEK) graft deturgescence in preservation medium after microkeratome cut using Fourier domain optical coherence tomography. Methods: The central and peripheral thickness of DSAEK grafts was measured by Fourier domain optical coherence tomography immediately after microkeratome cuts and 1, 2, 3, and 4 hours afterward. All measurements were taken when the grafts were stored in 4°C preservation medium. The hourly change in central graft thickness and graft shape (peripheral graft thicknes 2 central graft thickness) was calculated and tracked over time. Results: Five DSAEK grafts were measured. The average central graft thickness was 188.7 ± 44.4 μm (range, 146-255 μm) immediately after microkeratome cuts. The average central graft thickness was 147.5 ± 33.0 μm (range, 116-190 μm) after 4 hours in preservation medium (P <0.001). The average hourly change in central graft thickness was 230.5 mm (P = 0.0051), 28.6 mm (P = 0.055), 22.0 μm (P = 0.42), and 0.0 μm (P = 0.93) at 1, 2, 3, and 4 hours, respectively, after microkeratome cuts. The average hourly change in graft shape was insignificant. Conclusions: DSAEK grafts become thinner after microkeratome cut and stabilize at approximately 2 hours. Therefore, DSAEK graft thickness should be measured at 1.5 to 3 hours after microkeratome cut.",
keywords = "Corneal deturgescence, Eye bank, Optical coherence tomography",
author = "Maolong Tang and Christopher Stoeger and Joshua Galloway and Jeffrey Holiman and Bald, {Matthew R.} and David Huang",
year = "2013",
month = "6",
doi = "10.1097/ICO.0b013e31828a27dd",
language = "English (US)",
volume = "32",
pages = "847--850",
journal = "Cornea",
issn = "0277-3740",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Evaluating DSAEK graft deturgescence in preservation medium after microkeratome cut with optical coherence tomography

AU - Tang, Maolong

AU - Stoeger, Christopher

AU - Galloway, Joshua

AU - Holiman, Jeffrey

AU - Bald, Matthew R.

AU - Huang, David

PY - 2013/6

Y1 - 2013/6

N2 - Purpose: To evaluate the Descemet stripping automated endothelial keratoplasty (DSAEK) graft deturgescence in preservation medium after microkeratome cut using Fourier domain optical coherence tomography. Methods: The central and peripheral thickness of DSAEK grafts was measured by Fourier domain optical coherence tomography immediately after microkeratome cuts and 1, 2, 3, and 4 hours afterward. All measurements were taken when the grafts were stored in 4°C preservation medium. The hourly change in central graft thickness and graft shape (peripheral graft thicknes 2 central graft thickness) was calculated and tracked over time. Results: Five DSAEK grafts were measured. The average central graft thickness was 188.7 ± 44.4 μm (range, 146-255 μm) immediately after microkeratome cuts. The average central graft thickness was 147.5 ± 33.0 μm (range, 116-190 μm) after 4 hours in preservation medium (P <0.001). The average hourly change in central graft thickness was 230.5 mm (P = 0.0051), 28.6 mm (P = 0.055), 22.0 μm (P = 0.42), and 0.0 μm (P = 0.93) at 1, 2, 3, and 4 hours, respectively, after microkeratome cuts. The average hourly change in graft shape was insignificant. Conclusions: DSAEK grafts become thinner after microkeratome cut and stabilize at approximately 2 hours. Therefore, DSAEK graft thickness should be measured at 1.5 to 3 hours after microkeratome cut.

AB - Purpose: To evaluate the Descemet stripping automated endothelial keratoplasty (DSAEK) graft deturgescence in preservation medium after microkeratome cut using Fourier domain optical coherence tomography. Methods: The central and peripheral thickness of DSAEK grafts was measured by Fourier domain optical coherence tomography immediately after microkeratome cuts and 1, 2, 3, and 4 hours afterward. All measurements were taken when the grafts were stored in 4°C preservation medium. The hourly change in central graft thickness and graft shape (peripheral graft thicknes 2 central graft thickness) was calculated and tracked over time. Results: Five DSAEK grafts were measured. The average central graft thickness was 188.7 ± 44.4 μm (range, 146-255 μm) immediately after microkeratome cuts. The average central graft thickness was 147.5 ± 33.0 μm (range, 116-190 μm) after 4 hours in preservation medium (P <0.001). The average hourly change in central graft thickness was 230.5 mm (P = 0.0051), 28.6 mm (P = 0.055), 22.0 μm (P = 0.42), and 0.0 μm (P = 0.93) at 1, 2, 3, and 4 hours, respectively, after microkeratome cuts. The average hourly change in graft shape was insignificant. Conclusions: DSAEK grafts become thinner after microkeratome cut and stabilize at approximately 2 hours. Therefore, DSAEK graft thickness should be measured at 1.5 to 3 hours after microkeratome cut.

KW - Corneal deturgescence

KW - Eye bank

KW - Optical coherence tomography

UR - http://www.scopus.com/inward/record.url?scp=84879175390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879175390&partnerID=8YFLogxK

U2 - 10.1097/ICO.0b013e31828a27dd

DO - 10.1097/ICO.0b013e31828a27dd

M3 - Article

C2 - 23538636

AN - SCOPUS:84879175390

VL - 32

SP - 847

EP - 850

JO - Cornea

JF - Cornea

SN - 0277-3740

IS - 6

ER -