Ethanol inhibits human osteoblastic cell proliferation

Robert Klein, Kristina A. Fausti, Amy S. Carlos

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The habitual consumption of alcoholic beverages is clearly associated with low bone mass and an increased prevalence of skeletal fractures. Microscopic analysis of skeletal tissue from alcoholic patients reveals reduced osteoblast number and suppressed bone formation activity with a relative sparing of resorptive indices. The decreased number of osteoblasts observed in alcoholic subjects results from either impaired proliferation or accelerated senescence. Polyamines and omithine decarboxylase (ODC), the rate-limiting enzyme for polyamine synthesis, are essential for cell proliferation in a variety of cell types. To determine if the adverse effect of ethanol on osteoblast number involves modulation of polyamine biosynthesis, we examined the effect of ethanol on parameters of cell growth and ODC activity in a human osteoblast-like osteosarcoma cell line (TE85). Ethanol markedly impaired DNA synthesis and cell proliferation in a dose- dependent fashion, but alkaline phosphatase activity (a marker of differentiated osteoblast function) remained intact, and accelerated apoptosis was not evident. Thus, the reduced osteoblastic cell number was a result of a direct effect on proliferative processes rather than a nonspecific toxic effect of ethanol to accelerate cell death. Induction of ODC activity was impaired in ethanol-exposed cell cultures in a dose- dependent fashion that paralleled the antiproliferative effects. Finally, supplemental polyamine administration substantially improved DNA synthesis in ethanol-exposed UMR 106-01 cell cultures. These data confirm a direct inhibitory effect of ethanol on osteoblast proliferation without overt cellular toxicity that may, in part, explain the reduced bone mass observed in those who consume excessive amounts of alcohol.

Original languageEnglish (US)
Pages (from-to)572-578
Number of pages7
JournalAlcoholism: Clinical and Experimental Research
Volume20
Issue number3
StatePublished - 1996

Fingerprint

Cell proliferation
Osteoblasts
Ethanol
Cell Proliferation
Polyamines
Carboxy-Lyases
Bone
Alcoholics
Cell culture
Cell Culture Techniques
Bone and Bones
Alcoholic Beverages
Poisons
DNA
Biosynthesis
Cell growth
Cell death
Osteosarcoma
Osteogenesis
Alkaline Phosphatase

Keywords

  • Ethanol
  • Ornithine Decarboxylase
  • Osteoblast
  • Osteoporosis
  • Polyamines

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Ethanol inhibits human osteoblastic cell proliferation. / Klein, Robert; Fausti, Kristina A.; Carlos, Amy S.

In: Alcoholism: Clinical and Experimental Research, Vol. 20, No. 3, 1996, p. 572-578.

Research output: Contribution to journalArticle

Klein, Robert ; Fausti, Kristina A. ; Carlos, Amy S. / Ethanol inhibits human osteoblastic cell proliferation. In: Alcoholism: Clinical and Experimental Research. 1996 ; Vol. 20, No. 3. pp. 572-578.
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