TY - JOUR
T1 - Ethanol drinking in Withdrawal Seizure-Prone and -Resistant selected mouse lines
AU - Crabbe, John C.
AU - Spence, Stephanie E.
AU - Huang, Lawrence C.
AU - Cameron, Andy J.
AU - Schlumbohm, Jason P.
AU - Barkley-Levenson, Amanda M.
AU - Metten, Pamela
N1 - Funding Information:
These studies were supported by Grants AA10760 , AA13519 , and AA20245 from the NIH-NIAAA ; by the Department of Veterans Affairs ; and by the Department of the Army/DoD-TATRC grant 10245005.05 . AMB-L was supported by NIH-NIAAA grant AA007468 , the Achievement Rewards for College Scientists Foundation, and an OHSU Graduate Research Scholar fellowship.
PY - 2013/8
Y1 - 2013/8
N2 - Withdrawal Seizure-Prone (WSP) and Withdrawal Seizure-Resistant (WSR) mouse lines were bidirectionally selectively bred, respectively, to have severe or mild ethanol withdrawal handling-induced convulsions (HICs) after cessation of 3 days of ethanol vapor inhalation. Murine genotypes with severe withdrawal have been found to show low ethanol consumption, and high consumers show low withdrawal. An early drinking study with WSP and WSR mice showed modest evidence consistent with this genetic correlation, but there were several limitations to that experiment. We therefore conducted a thorough assessment of two bottle ethanol preference drinking in both replicate pairs of WSP/WSR selected lines in mice of both sexes. Greater preference drinking of WSR-2 than WSP-2 female mice confirmed the earlier report. However, in the parallel set of selected lines, the WSP-1 mice drank more than the WSR-1s. Naive mice tested for preference for sucrose, saccharin and quinine did not differ markedly for any tastant. Finally, in a test of binge-like drinking, Drinking in the Dark (DID), WSP mice drank more than WSR mice and attained significantly higher (but still modest) blood ethanol concentrations. Tests of acute withdrawal after DID showed a mild, but significant elevation in handling-induced convulsions in the WSP line. These results provide further evidence that 2-bottle ethanol preference and DID are genetically distinguishable traits.
AB - Withdrawal Seizure-Prone (WSP) and Withdrawal Seizure-Resistant (WSR) mouse lines were bidirectionally selectively bred, respectively, to have severe or mild ethanol withdrawal handling-induced convulsions (HICs) after cessation of 3 days of ethanol vapor inhalation. Murine genotypes with severe withdrawal have been found to show low ethanol consumption, and high consumers show low withdrawal. An early drinking study with WSP and WSR mice showed modest evidence consistent with this genetic correlation, but there were several limitations to that experiment. We therefore conducted a thorough assessment of two bottle ethanol preference drinking in both replicate pairs of WSP/WSR selected lines in mice of both sexes. Greater preference drinking of WSR-2 than WSP-2 female mice confirmed the earlier report. However, in the parallel set of selected lines, the WSP-1 mice drank more than the WSR-1s. Naive mice tested for preference for sucrose, saccharin and quinine did not differ markedly for any tastant. Finally, in a test of binge-like drinking, Drinking in the Dark (DID), WSP mice drank more than WSR mice and attained significantly higher (but still modest) blood ethanol concentrations. Tests of acute withdrawal after DID showed a mild, but significant elevation in handling-induced convulsions in the WSP line. These results provide further evidence that 2-bottle ethanol preference and DID are genetically distinguishable traits.
KW - Drinking in the dark
KW - Ethanol preference
KW - Ethanol withdrawal
KW - Genetics
KW - Mouse
KW - Selective breeding
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U2 - 10.1016/j.alcohol.2013.05.002
DO - 10.1016/j.alcohol.2013.05.002
M3 - Article
C2 - 23809872
AN - SCOPUS:84879728341
SN - 0741-8329
VL - 47
SP - 381
EP - 389
JO - Alcohol
JF - Alcohol
IS - 5
ER -