Ethanol drinking in Withdrawal Seizure-Prone and -Resistant selected mouse lines

John Jr Crabbe, Stephanie E. Spence, Lawrence C. Huang, Andy J. Cameron, Jason P. Schlumbohm, Amanda M. Barkley-Levenson, Pamela Metten

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Withdrawal Seizure-Prone (WSP) and Withdrawal Seizure-Resistant (WSR) mouse lines were bidirectionally selectively bred, respectively, to have severe or mild ethanol withdrawal handling-induced convulsions (HICs) after cessation of 3 days of ethanol vapor inhalation. Murine genotypes with severe withdrawal have been found to show low ethanol consumption, and high consumers show low withdrawal. An early drinking study with WSP and WSR mice showed modest evidence consistent with this genetic correlation, but there were several limitations to that experiment. We therefore conducted a thorough assessment of two bottle ethanol preference drinking in both replicate pairs of WSP/WSR selected lines in mice of both sexes. Greater preference drinking of WSR-2 than WSP-2 female mice confirmed the earlier report. However, in the parallel set of selected lines, the WSP-1 mice drank more than the WSR-1s. Naive mice tested for preference for sucrose, saccharin and quinine did not differ markedly for any tastant. Finally, in a test of binge-like drinking, Drinking in the Dark (DID), WSP mice drank more than WSR mice and attained significantly higher (but still modest) blood ethanol concentrations. Tests of acute withdrawal after DID showed a mild, but significant elevation in handling-induced convulsions in the WSP line. These results provide further evidence that 2-bottle ethanol preference and DID are genetically distinguishable traits.

Original languageEnglish (US)
Pages (from-to)381-389
Number of pages9
JournalAlcohol
Volume47
Issue number5
DOIs
StatePublished - Aug 2013

Fingerprint

seizure
withdrawal
Drinking
Seizures
Ethanol
Bottles
Saccharin
Quinine
Sucrose
Blood
Vapors
Binge Drinking
Experiments
Inhalation

Keywords

  • Drinking in the dark
  • Ethanol preference
  • Ethanol withdrawal
  • Genetics
  • Mouse
  • Selective breeding

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neurology
  • Toxicology
  • Health(social science)

Cite this

Crabbe, J. J., Spence, S. E., Huang, L. C., Cameron, A. J., Schlumbohm, J. P., Barkley-Levenson, A. M., & Metten, P. (2013). Ethanol drinking in Withdrawal Seizure-Prone and -Resistant selected mouse lines. Alcohol, 47(5), 381-389. https://doi.org/10.1016/j.alcohol.2013.05.002

Ethanol drinking in Withdrawal Seizure-Prone and -Resistant selected mouse lines. / Crabbe, John Jr; Spence, Stephanie E.; Huang, Lawrence C.; Cameron, Andy J.; Schlumbohm, Jason P.; Barkley-Levenson, Amanda M.; Metten, Pamela.

In: Alcohol, Vol. 47, No. 5, 08.2013, p. 381-389.

Research output: Contribution to journalArticle

Crabbe, JJ, Spence, SE, Huang, LC, Cameron, AJ, Schlumbohm, JP, Barkley-Levenson, AM & Metten, P 2013, 'Ethanol drinking in Withdrawal Seizure-Prone and -Resistant selected mouse lines', Alcohol, vol. 47, no. 5, pp. 381-389. https://doi.org/10.1016/j.alcohol.2013.05.002
Crabbe JJ, Spence SE, Huang LC, Cameron AJ, Schlumbohm JP, Barkley-Levenson AM et al. Ethanol drinking in Withdrawal Seizure-Prone and -Resistant selected mouse lines. Alcohol. 2013 Aug;47(5):381-389. https://doi.org/10.1016/j.alcohol.2013.05.002
Crabbe, John Jr ; Spence, Stephanie E. ; Huang, Lawrence C. ; Cameron, Andy J. ; Schlumbohm, Jason P. ; Barkley-Levenson, Amanda M. ; Metten, Pamela. / Ethanol drinking in Withdrawal Seizure-Prone and -Resistant selected mouse lines. In: Alcohol. 2013 ; Vol. 47, No. 5. pp. 381-389.
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