Ethanol-conditioned place preference is reduced in dopamine D2 receptor-deficient mice

Christopher Cunningham, MacKenzie A. Howard, Sylvia J. Gill, Marcelo Rubinstein, Malcolm J. Low, David Grandy

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Pharmacological blockade studies have supported a role of the dopamine system in ethanol reward for many years, but receptor subtype specificity has been difficult to establish. Recently, genetically engineered mice lacking functional dopamine D2 receptors have been shown to drink less ethanol in a two-bottle choice task. To determine whether reduced ethanol intake reflects a reduction in ethanol reward, D2 receptor-deficient [knockout (KO)] mice were compared to heterozygous (HET) and wild-type (WT; C57BL/6 × DBA/2 F2 hybrid) mice in a place conditioning task. Under conditions that produced reliable place preference in both WT and HET mice, KO mice showed no evidence of place conditioning, suggesting that D2 receptor gene inactivation reduced ethanol reward or the ability to learn about ethanol reward. Consistent with previous findings, this mutation also produced a gene dose-related reduction in basal activity levels. Moreover, KO and HET mice showed enhancement of ethanol-stimulated activity relative to WT mice. However, differences in basal and ethanol-stimulated activity did not explain the differences in place conditioning. Overall, this study strongly supports the conclusion that dopamine D2 receptors normally influence ethanol reward in mice.

Original languageEnglish (US)
Pages (from-to)693-699
Number of pages7
JournalPharmacology Biochemistry and Behavior
Volume67
Issue number4
DOIs
StatePublished - 2000

Fingerprint

Dopamine D2 Receptors
Ethanol
Reward
Knockout Mice
Genes
mouse DRD2 protein
Aptitude
Bottles
Gene Silencing
Dopamine
Pharmacology
Mutation

Keywords

  • C57BL/6×DBA/2 F2 hybrid mice
  • Conditioned place preference
  • Dopamine D2 receptor
  • Ethanol
  • Knockout mice
  • Locomotor activity

ASJC Scopus subject areas

  • Biochemistry
  • Behavioral Neuroscience
  • Pharmacology

Cite this

Ethanol-conditioned place preference is reduced in dopamine D2 receptor-deficient mice. / Cunningham, Christopher; Howard, MacKenzie A.; Gill, Sylvia J.; Rubinstein, Marcelo; Low, Malcolm J.; Grandy, David.

In: Pharmacology Biochemistry and Behavior, Vol. 67, No. 4, 2000, p. 693-699.

Research output: Contribution to journalArticle

Cunningham, Christopher ; Howard, MacKenzie A. ; Gill, Sylvia J. ; Rubinstein, Marcelo ; Low, Malcolm J. ; Grandy, David. / Ethanol-conditioned place preference is reduced in dopamine D2 receptor-deficient mice. In: Pharmacology Biochemistry and Behavior. 2000 ; Vol. 67, No. 4. pp. 693-699.
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