Estrogen receptors and aromatase activity in the hypothalamus of the female frog, Rana esculenta. Fluctuations throughout the reproductive cycle

Giulia Guerriero, Charles E. Roselli, Marina Paolucci, Virgilio Botte, Gaetano Ciarcia

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

It is well known that certain actions of androgen are mediated through in situ aromatization to estrogen in neural target tissues. This study was undertaken to investigate androgen utilization in the hypothalamus of the female frog, Rana esculenta, through a quantification of estrogen receptors and aromatase activity during the reproductive cycle. 3H-estradiol-binding molecules were present in both the cytosol and the nuclear extract of the hypothalamus. These molecules bound specifically 3H-estradiol with high affinity (Kd 10-10 M) and low capacity (cytosol: 1.2±0.4 fmol/mg protein; nuclear extract: 7.9±0.6 fmol/mg protein). Aromatase activity was detected in the microsomal fraction of the hypothalamus using a sensitive in vitro radiometric assay. Both aromatase activity and nuclear estrogen receptor binding fluctuated in synchrony throughout the reproductive cycle. Western blot analysis of aromatase protein revealed one immunoreactive band with a molecular weight of approximately 56 kDa. In contrast to aromatase enzyme activity, the relative levels of aromatase protein changed little during the reproductive cycle suggesting that post-translational mechanisms may be involved in regulating estrogen synthesis in the frog brain. A possible role for estrogens in the modulation of the reproductive behavior in this species is suggested. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)92-101
Number of pages10
JournalBrain research
Volume880
Issue number1-2
DOIs
StatePublished - Oct 13 2000

Keywords

  • Aromatase
  • Estrogen receptors
  • Frog
  • Hypothalamus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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